Schmidt Christopher W, Moore Christopher R, Leifheit Randell
Indiana Prehistory Laboratory, Department of Anthropology, University of Indianapolis, Indianapolis, IN, USA.
Methods Mol Biol. 2012;915:235-48. doi: 10.1007/978-1-61779-977-8_14.
White light confocal microscopy creates detailed 3D representations of microsurfaces that can be qualitatively and quantitatively analyzed. The study describes its application to the analysis of cut marks on bone, particularly when discerning cuts made by steel tools from those made by stone. The process described comes from a study where cuts were manually made on a cow rib with seven cutting tools, four stone (an unmodified chert flake, a chert biface, a bifacially ground slate fragment, and an unsharpened piece of slate), and three steel (a Swiss Army Knife, a serrate steak knife, and a serrate saw). Kerfs were magnified ×20 and 3D data clouds were generated using a Sensofar(®) White Light Confocal Profiler (WLCP). Kerf profiles and surface areas, volumes, mean depths, and maximum depths were calculated with proprietary software (SensoScan(®) and SolarMap(®)). For the most part, the stone tools make shallower and wider cuts. Kerf floors can be studied at higher magnifications; they were viewed at ×100. When comparing the kerf floors of the unsharpened slate and the serrate steak knife it was found that the slate floor was more uneven, but the serrate steak knife generated more overall relief. Although preliminary, the approach described here successfully distinguishes stone and steel tools; the authors conclude that the WLCP is a promising technology for cut mark analysis because of the very detailed 3D representations it creates and the numerous avenues of analysis it provides.
白光共聚焦显微镜可创建微表面的详细三维图像,这些图像可进行定性和定量分析。该研究描述了其在骨头上切割痕迹分析中的应用,特别是在区分钢制工具和石制工具所造成的切割痕迹时。所描述的过程源自一项研究,在该研究中,使用七种切割工具在一根牛肋骨上进行手动切割,其中四种是石制工具(一块未加工的燧石薄片、一把燧石双面器、一块双面打磨的板岩碎片和一块未打磨的板岩),三种是钢制工具(一把瑞士军刀、一把锯齿牛排刀和一把锯齿锯)。切口被放大20倍,并使用Sensofar(®)白光共聚焦轮廓仪(WLCP)生成三维数据云。使用专有软件(SensoScan(®)和SolarMap(®))计算切口轮廓以及表面积、体积、平均深度和最大深度。在大多数情况下,石制工具造成的切口更浅且更宽。可以在更高放大倍数下研究切口底部;切口底部以100倍放大倍数观察。在比较未打磨板岩和锯齿牛排刀的切口底部时发现,板岩底部更不平整,但锯齿牛排刀产生的整体起伏更大。尽管是初步研究,但这里描述的方法成功地区分了石制工具和钢制工具;作者得出结论,由于白光共聚焦轮廓仪创建的三维图像非常详细且提供了众多分析途径,因此它是一种很有前景的切割痕迹分析技术。