Lin Ting-Yu, Chien Shih-Chang, Kuo Yueh-Hsiung, Wang Sheng-Yang
Department of Forestry, National Chung-Hsing University, Taichung, Taiwan.
Nat Prod Commun. 2012 Jul;7(7):835-6.
Antcin C is a bioactive compound isolated from Antrodia cinnamomea, a rare and expensive medicinal fungus endemic to Taiwan. Up to date, the absolute structure of antcin C has not been solved. In this study, the phenylglycine methyl ester (PGME) derivatives of antcin C were prepared. From NMR analysis the absolute configuration of(S)-antcin C (1) and (R)-antcin C (2) were determined. By MTT assay, (S)-antcin C presented cytotoxicity against Hep G2 and MCF-7 cells, with IC50 values of 14.5 and 12.8 microg/mL, respectively. However, (R)-antcin C did not show significant cytotoxicity. Our results showed that the configuration at C25 for antcin C is very important for its cytotoxicity. From a quality control point of view, it is necessary to identify and quantify the R- and S- enantiomers in the A. cinnamomea products.
安替新C是从台湾特有的一种珍稀且昂贵的药用真菌——樟芝中分离出的一种生物活性化合物。截至目前，安替新C的绝对结构尚未得到解析。在本研究中，制备了安替新C的苯甘氨酸甲酯（PGME）衍生物。通过核磁共振分析确定了（S）-安替新C（1）和（R）-安替新C（2）的绝对构型。通过MTT法检测，（S）-安替新C对肝癌细胞Hep G2和乳腺癌细胞MCF-7呈现细胞毒性，IC50值分别为14.5和12.8微克/毫升。然而，（R）-安替新C未显示出明显的细胞毒性。我们的结果表明，安替新C在C25位的构型对其细胞毒性非常重要。从质量控制的角度来看，有必要对樟芝产品中的R-和S-对映体进行鉴定和定量。
