Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung 404, Taiwan.
Int J Mol Sci. 2013 Feb 26;14(3):4629-39. doi: 10.3390/ijms14034629.
Three new benzenoids, 3-isopropenyl-2-methoxy-6-methyl-4,5-methylenedioxy- phenol (1), 2-hydroxy-4,4'-dimethoxy-3,3'-dimethyl-5,6,5',6'-bimethylenedioxybiphenyl (2), 4,4'-dihydroxy-3,3'-dimethoxy-2,2'-dimethyl-5,6,5',6'-bimethylenedioxybiphenyl (3), together with two known benzenoids, 2,3,6-trimethoxy-5-methylphenol (4) and 2,3-methylenedioxy- 4-methoxy-5-methylphenol (5), were isolated from Antrodia camphorata. Our results support that compounds 1-5 potently inhibited LPS (lipopolysaccharide)-induced nitric oxide (NO) production in a dose-dependent manner. The IC(50) values of compounds 1, 3 and 5 were 1.8 ± 0.2, 18.8 ± 0.6 and 0.8 ± 0.3 μg/mL, respectively.
3-异丙烯基-2-甲氧基-6-甲基-4,5-亚甲二氧基苯酚（1）、2-羟基-4,4′-二甲氧基-3,3′-二甲基-5,6,5′,6′-双亚甲二氧基联苯（2）、4,4′-二羟基-3,3′-二甲氧基-2,2′-二甲基-5,6,5′,6′-双亚甲二氧基联苯（3），以及两种已知的苯丙素类化合物：2,3,6-三甲氧基-5-甲基苯酚（4）和 2,3-亚甲二氧基-4-甲氧基-5-甲基苯酚（5）。我们的结果表明，化合物 1-5 能够以剂量依赖的方式强烈抑制 LPS（脂多糖）诱导的 NO（一氧化氮）产生。化合物 1、3 和 5 的 IC50 值分别为 1.8 ± 0.2、18.8 ± 0.6 和 0.8 ± 0.3 μg/mL。
