The Beatson Institute for Cancer Research, Garscube Estate, Switchback Rd., Bearsden, Glasgow G61 1BD, UK.
Biochem J. 2012 Nov 15;448(1):35-42. doi: 10.1042/BJ20112206.
The Scar (suppressor of cAMP receptor)/WAVE [WASP (Wiskott-Aldrich syndrome protein) verprolin homologous] complex plays a major role in the motility of cells by activating the Arp2/3 complex, which initiates actin branching and drives protrusions. Mammals have three Scar/WAVE isoforms, which show some tissue-specific expression, but their functions have not been differentiated. In the present study we show that depletion of Scar/WAVE3 in the mammalian breast cancer cells MDA-MB-231 results in larger and less dynamic lamellipodia. Scar/WAVE3-depleted cells move more slowly but more persistently on a two-dimensional matrix and they typically only show one lamellipod. However, Scar/WAVE3 appears to have no role in driving invasiveness in a three-dimensional Matrigel™ invasion assay or a three-dimensional collagen invasion assay, suggesting that lamellipodial persistence as seen in two-dimensions is not crucial in three-dimensional environments.
疤痕（环腺苷酸受体）/WAVE [WASP（威特综合征蛋白）verprolin 同源物]复合物通过激活 Arp2/3 复合物在细胞运动中发挥主要作用，该复合物引发肌动蛋白分支并驱动突起。哺乳动物有三种 Scar/WAVE 同工型，它们表现出一些组织特异性表达，但它们的功能尚未分化。在本研究中，我们表明 Scar/WAVE3 在乳腺癌细胞 MDA-MB-231 中的耗竭导致更大和动态性更小的片状伪足。Scar/WAVE3 耗竭的细胞在二维基质上移动更慢但更持久，它们通常只显示一个片状伪足。然而，Scar/WAVE3 似乎在三维 Matrigel™侵袭测定或三维胶原侵袭测定中没有驱动侵袭的作用，表明二维观察到的片状伪足的持久性在三维环境中并不重要。
