疤痕/波浪 3 有助于片状伪足动力学的运动性和可塑性，但不影响三维入侵。
Scar/WAVE3 contributes to motility and plasticity of lamellipodial dynamics but not invasion in three dimensions.
机构信息
The Beatson Institute for Cancer Research, Garscube Estate, Switchback Rd., Bearsden, Glasgow G61 1BD, UK.
出版信息
Biochem J. 2012 Nov 15;448(1):35-42. doi: 10.1042/BJ20112206.
Abstract
The Scar (suppressor of cAMP receptor)/WAVE [WASP (Wiskott-Aldrich syndrome protein) verprolin homologous] complex plays a major role in the motility of cells by activating the Arp2/3 complex, which initiates actin branching and drives protrusions. Mammals have three Scar/WAVE isoforms, which show some tissue-specific expression, but their functions have not been differentiated. In the present study we show that depletion of Scar/WAVE3 in the mammalian breast cancer cells MDA-MB-231 results in larger and less dynamic lamellipodia. Scar/WAVE3-depleted cells move more slowly but more persistently on a two-dimensional matrix and they typically only show one lamellipod. However, Scar/WAVE3 appears to have no role in driving invasiveness in a three-dimensional Matrigel™ invasion assay or a three-dimensional collagen invasion assay, suggesting that lamellipodial persistence as seen in two-dimensions is not crucial in three-dimensional environments.
摘要
疤痕（环腺苷酸受体）/WAVE [WASP（威特综合征蛋白）verprolin 同源物]复合物通过激活 Arp2/3 复合物在细胞运动中发挥主要作用，该复合物引发肌动蛋白分支并驱动突起。哺乳动物有三种 Scar/WAVE 同工型，它们表现出一些组织特异性表达，但它们的功能尚未分化。在本研究中，我们表明 Scar/WAVE3 在乳腺癌细胞 MDA-MB-231 中的耗竭导致更大和动态性更小的片状伪足。Scar/WAVE3 耗竭的细胞在二维基质上移动更慢但更持久，它们通常只显示一个片状伪足。然而，Scar/WAVE3 似乎在三维 Matrigel™侵袭测定或三维胶原侵袭测定中没有驱动侵袭的作用，表明二维观察到的片状伪足的持久性在三维环境中并不重要。
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