Nasim Sadia, Shahid Anjum, Mustufa Muhammad Ayaz, Arain Ghazala Mohyuddin, Ali Ghazanfer, Taseer Ijaz-ul-Haque, Talreja Kanaya Lal, Firdous Rukhsana, Iqbal Rizwan, Siddique Shameem Ahmed, Naz Saima, Akhter Tasleem
Pakistan Medical Research Council, Specialized Research Centre on Child Health, National Institute of Child Health, Karachi, Pakistan.
J Infect Dev Ctries. 2012 Aug 21;6(8):611-9. doi: 10.3855/jidc.2236.
Biosafety during lab work is an important concern in developing countries. Some critical issues concerning biosafety are lack of training, exceeding workload, working too fast, deciding not to follow safe practices, and skepticism about biohazards. This study aimed to determine biosafety perception and practices of laboratory technicians during routine work in clinical laboratories of Pakistan.
A total of 1,782 laboratory technicians were interviewed from major public sector hospitals and a few private hospital laboratories throughout Pakistan.
A total of 1,647 (92.4%) males and 135 (7.6%) females participated in the study, with over half (59.7%) having more than five years of work experience. Results showed that 28.4% of the laboratory technicians from Punjab, 35.7% from Sindh, 32% from Balochistan and 38.4% from Khyber Pakhtoon Khawa (KPK) did not use any personal protective equipment. Almost 46% of the respondents (34.2% from Punjab, 61.9% from Sindh, 25.2% from Balochistan and 85% from KPK) said they reused syringes either occasionally or regularly. Furthermore, 30.7% of the respondents said they discard used syringes directly into municipal dustbins. The majority (66.7%) claimed there are no separate bins for sharps, so they throw these in municipal dustbins. Mouth pipetting was reported by 28.3% technicians. Standard operating procedures were not available in 67.2% labs, and accident records were not maintained in 83.4%. No formal biosafety training had been provided to 84.2% of the respondents.
Laboratory technicians in Pakistan lack awareness of good laboratory practices and biosafety measures, and also face a lack of resources.
在发展中国家，实验室工作中的生物安全是一个重要问题。一些与生物安全相关的关键问题包括缺乏培训、工作量过大、工作速度过快、决定不遵循安全操作规程以及对生物危害持怀疑态度。本研究旨在确定巴基斯坦临床实验室的实验室技术人员在日常工作中的生物安全认知和实践情况。
从巴基斯坦各地的主要公共部门医院和一些私立医院实验室共采访了1782名实验室技术人员。
共有1647名（92.4%）男性和135名（7.6%）女性参与了该研究，其中超过一半（59.7%）拥有五年以上工作经验。结果显示，旁遮普邦28.4%的实验室技术人员、信德省35.7%的实验室技术人员、俾路支省32%的实验室技术人员以及开伯尔-普赫图赫瓦省（KPK）38.4%的实验室技术人员未使用任何个人防护设备。近46%的受访者（旁遮普邦为34.2%、信德省为61.9%、俾路支省为25.2%、开伯尔-普赫图赫瓦省为85%）表示他们偶尔或经常重复使用注射器。此外，30.7%的受访者表示他们将用过的注射器直接丢弃到城市垃圾桶中。大多数（66.7%）人称没有专门的锐器盒，所以他们将这些物品扔到城市垃圾桶中。28.3%的技术人员报告有口吸操作。67.2%的实验室没有标准操作规程，83.4%的实验室没有事故记录。84.2%的受访者未接受过正规的生物安全培训。
巴基斯坦的实验室技术人员缺乏良好实验室规范和生物安全措施的意识，并且还面临资源短缺的问题。
