Institute for Biomedical Technology and Technical Medicine, University of Twente, AE Enschede, The Netherlands.
Electrophoresis. 2012 Sep;33(18):2892-5. doi: 10.1002/elps.201200275. Epub 2012 Aug 22.
This work presents the application of membrane technology for the fabrication of stationary phase for CEC columns using the technique based on phase inversion of polymer solution. A blend of polyimide P84 and sulphonated poly(ether ether ketone was processed via immersion precipitation dry-wet spinning into small-bore porous fiber. The morphology, zeta potential, and performance of the porous structure in the CEC separation were investigated. Noncharged molecules (as markers of the electroosmotic flow) and small organic compounds were injected into the column, driven under the application of voltage, and detected on the electropherogram. The proof of concept of applying porous membrane structure as stationary phase for CEC was shown and possible optimization to improve efficiency and selectivity was suggested.
本工作介绍了膜技术在使用基于聚合物溶液相转化技术制备 CEC 柱固定相方面的应用。聚酰亚胺 P84 和磺化聚醚醚酮的混合物通过浸没沉淀干湿纺工艺加工成小内径多孔纤维。研究了 CEC 分离中多孔结构的形态、zeta 电位和性能。将非带电分子（作为电渗流的标志物）和小分子有机化合物注入柱中，在施加电压的情况下驱动，并在电泳图上检测。证明了将多孔膜结构作为 CEC 的固定相的概念，并提出了可能的优化方案，以提高效率和选择性。
