Hacettepe University, Faculty of Pharmacy, Department of Basic Pharmaceutical Sciences, 06100 Ankara, Turkey.
J Chromatogr A. 2010 Mar 5;1217(10):1654-9. doi: 10.1016/j.chroma.2010.01.020. Epub 2010 Jan 18.
The use of high internal phase emulsion polymers (polyHIPEs) for CEC applications has remained relatively unexplored. A few reports exist in the literature for the preparation of similar structures. In this study, polyHIPEs having high porosity, and interconnected open-cell structure, were introduced and evaluated as stationary phase for CEC. The polyHIPE monolithic columns were prepared by the in situ polymerization of isodecylacrylate (IDA) and divinylbenzene (DVB) in the continuous phase of a high internal phase emulsion (HIPE). Due to its well-defined polyHIPE structure with interconnected micron size spherical voids, the columns synthesized with different initiator concentrations were successfully used for the separation of alkylbenzenes. Furthermore, the columns indicated a strong electroosmotic flow (EOF) without any additional EOF generating monomer probably due to the presence of ionizable sulfate groups coming from the water-soluble initiator used in the preparation of polyHIPE matrix. The best chromatographic performance in the separation of alkylbenzenes was achieved by using 70% ACN in the mobile phase with high column efficiency (up to 200,000 plates/m).
用于 CEC 应用的高内相比乳液聚合物 (polyHIPE) 的使用仍相对未被探索。文献中有一些关于制备类似结构的报道。在这项研究中，引入了具有高孔隙率和相互连通的开孔结构的 polyHIPE，并将其评估为 CEC 的固定相。通过在高内相比乳液 (HIPE) 的连续相中原位聚合异癸基丙烯酸酯 (IDA) 和二乙烯基苯 (DVB) 制备了 polyHIPE 整体柱。由于其具有相互连接的微米级球形空隙的明确定义的 polyHIPE 结构，因此用不同引发剂浓度合成的柱子成功地用于分离烷基苯。此外，由于制备 polyHIPE 基质中使用的水溶性引发剂中存在可离子化的硫酸根基团，柱子表现出强烈的电动流动 (EOF)，而无需任何额外的 EOF 生成单体。在分离烷基苯时，使用 70% ACN 作为流动相可获得最佳的色谱性能，柱效率高达 200,000 板/m。
