Zheng J Y, Wang H, Chen X X, Wang P, Gao P, Li X N, Zhu G P
Key Laboratory of Molecular Evolution and Biodiversity, Institute of Molecular Biology and Biotechnology, Anhui Normal University, Wuhu, P.R. China.
Genet Mol Res. 2012 Aug 6;11(3):1975-80. doi: 10.4238/2012.August.6.1.
Paris polyphylla var. chinensis is a perennial herb with medicinal properties that is widely used in traditional Chinese medicine. However, this plant has been on the edge of extinction during the last few decades because of excessive deforestation based on the intense ethnopharmaceutical interest. We isolated 12 microsatellite loci from a (CT)(n)-enriched genomic library of P. polyphylla var. chinensis. The polymorphism at each locus was analyzed by screening 30 individuals from a natural population. The number of alleles ranged from 2 to 5. The observed and expected heterozygosities ranged from 0.000 to 0.467, with a mean of 0.247, and from 0.383 to 0.662 with a mean of 0.537, respectively. Six loci (Pp1, Pp3, Pp6, Pp7, Pp9, and Pp12) were found to significantly deviate from Hardy-Weinberg equilibrium. This may be due to the small population size, inbreeding or null alleles. Five of the pairwise comparisons (Pp1 and Pp4, Pp2 and Pp5, Pp2 and Pp9, Pp2 and Pp12, Pp11 and Pp12) exhibited significant linkage disequilibrium (P < 0.05). We conclude that these microsatellite markers will be useful for population genetic studies of P. polyphylla var. chinensis.
华重楼是一种具有药用价值的多年生草本植物，在传统中药中广泛应用。然而，由于基于强烈的民族药学兴趣而进行的过度森林砍伐，这种植物在过去几十年里一直处于灭绝边缘。我们从华重楼的一个富含(CT)(n)的基因组文库中分离出12个微卫星位点。通过对一个自然种群的30个个体进行筛选，分析了每个位点的多态性。等位基因数量从2到5不等。观察到的杂合度和期望杂合度分别从0.000到0.467（平均为0.247）以及从0.383到0.662（平均为0.537）。发现6个位点（Pp1、Pp3、Pp6、Pp7、Pp9和Pp12）显著偏离哈迪-温伯格平衡。这可能是由于种群规模小、近亲繁殖或无效等位基因所致。成对比较中的5组（Pp1和Pp4、Pp2和Pp5、Pp2和Pp9、Pp2和Pp12、Pp11和Pp12）表现出显著的连锁不平衡（P < 0.05）。我们得出结论，这些微卫星标记将有助于对华重楼的种群遗传学研究。
