College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
Poult Sci. 2012 Sep;91(9):2121-9. doi: 10.3382/ps.2011-02131.
Four hundred and fifty 1-d-old male Lingnan Yellow broiler chickens were used to investigate the effects of Clostridium butyricum on growth performance, immune function, and cecal microflora. The birds were randomly assigned to 5 treatments and offered the same antibiotic-free basal diets for 42 d. The treatments were as follows: no addition (control), 1 × 10(7) cfu C. butyricum/kg of diet (CB1), 2 × 10(7) cfu C. butyricum/kg of diet (CB2), 3 × 10(7) cfu C. butyricum/kg of diet (CB3), and 10 mg of colistine sulfate/kg of diet (antibiotic). Birds fed either CB2 or antibiotic had greater overall BW than those in the control group. During d 1 to 7, d 21 to 42, and d 1 to 42, birds fed either CB2 or CB3 or the antibiotic diet had greater ADG compared with those in the control group. No significant differences were observed in BW or ADG among the CB2, CB3, and antibiotic groups. Birds fed the CB2 or CB3 diet had greater concentrations of IgA and IgG in the serum from d 14 to 42 and greater IgM in the serum from d 21 to 42 than those in the control group. Birds fed the CB3 diet had a greater concentration of complement component 3 in the serum than those in the control group from d 7 to 42. Dietary C. butyricum decreased (P < 0.05) Escherichia coli in cecal contents on d 14 and 42, and both CB2 and CB3 decreased (P < 0.05) cecal Salmonella and Clostridium perfringen from d 14 to 42 compared with the control. Broilers fed either CB2 or CB3 had greater cecal Lactobacillus and Bifidobacterium counts from d 21 to 42, and birds fed C. butyricum had greater cecal C. butyricum counts during the whole period compared with those in the control group. The results indicate that C. butyricum promotes growth performance and immune function and benefits the balance of the intestinal microflora in broiler chickens.
四百五十只 1 日龄岭南黄肉鸡用于研究丁酸梭菌对生长性能、免疫功能和盲肠微生物区系的影响。这些鸡被随机分为 5 组，在 42 天内提供相同的无抗生素基础日粮。处理如下：不添加（对照）、日粮中添加 1×10(7)cfu 丁酸梭菌/千克（CB1）、日粮中添加 2×10(7)cfu 丁酸梭菌/千克（CB2）、日粮中添加 3×10(7)cfu 丁酸梭菌/千克（CB3）和 10mg 硫酸粘菌素/千克日粮（抗生素）。饲喂 CB2 或抗生素的鸡在整个试验期间的 BW 均大于对照组。在第 1 至 7 天、第 21 至 42 天和第 1 至 42 天期间，饲喂 CB2 或 CB3 或抗生素日粮的鸡的 ADG 均大于对照组。CB2、CB3 和抗生素组之间的 BW 或 ADG 无显著差异。从第 14 天到第 42 天，饲喂 CB2 或 CB3 日粮的鸡血清中 IgA 和 IgG 浓度较高，从第 21 天到第 42 天，血清中 IgM 浓度较高，而对照组鸡血清中 IgA、IgG 和 IgM 浓度均无显著差异。饲喂 CB3 日粮的鸡血清中补体成分 3 的浓度从第 7 天到第 42 天比对照组高。日粮丁酸梭菌降低了（P＜0.05）第 14 天和第 42 天盲肠内容物中的大肠杆菌数量，且 CB2 和 CB3 均降低了（P＜0.05）第 14 天至第 42 天盲肠中沙门氏菌和产气荚膜梭菌数量。从第 21 天到第 42 天，饲喂 CB2 或 CB3 的鸡盲肠中乳酸菌和双歧杆菌数量增加，整个试验期间，饲喂丁酸梭菌的鸡盲肠中丁酸梭菌数量高于对照组。结果表明，丁酸梭菌能促进肉鸡生长性能和免疫功能，有利于维持肉鸡盲肠微生物菌群平衡。
