Department of Nutritional Physiology and Feeding, Agricultural University of Athens, Iera Odos 75, 11855, Greece.
Poult Sci. 2010 Jan;89(1):58-67. doi: 10.3382/ps.2009-00308.
The aim of this work was to investigate the effect of inclusion levels of a 5-bacterial species probiotic in broiler nutrition. Five hundred twenty-five 1-d-old male Cobb broilers were allocated in 5 experimental treatments for 6 wk. The experimental treatments received a corn-soybean coccidiostat-free basal diet and depending on the addition were labeled as follows: no addition (C), 10(8) cfu probiotic/kg of diet (P1), 10(9) cfu probiotic/kg of diet (P2), 10(10) cfu probiotic/kg of diet (P3), and 2.5 mg of avilamycin/kg of diet (A). Each treatment had 3 replicates of 35 broilers each. Treatment effects on broiler growth performance and biomarkers such as ileal and total tract nutrient digestibility, plasma Ig concentration, and cecal microflora composition were determined. Differences among treatments were considered significant when P < or = 0.05. Overall BW gain was significantly higher in treatment P1 (2,293 g) compared with P2 (2,163 g), C (2,165 g), and P3 (2,167 g), with A (2,230 g) being intermediate and not different from P1. Overall feed conversion ratio values were similar and significantly better for P1 (1.80) and A (1.80) compared with P2 (1.87), C (1.89), and P3 (1.92). Ileal apparent digestibility coefficients (ADC) of CP and ether extract were higher in A. Generally, treatments A and P1 showed an improved total tract ADC for DM, organic matter, ash, ether extract, and AME(n) values. The total tract ADC of CP was higher in P1, C, and P2. There were no differences between treatments regarding plasma Ig in 14- and 42-d-old broilers. Treatments P2 and P3 were effective at beneficially modulating cecal microflora composition. In particular, the lower cecal coliform concentration (log cfu/g of wet digesta) was seen in P2 (6.12) and P3 (4.90) in 14- and 42-d-old broilers, respectively, whereas at 42 d, P3 and P2 had the highest Bifidobacterium (8.31; 8.08) and Lactobacillus concentrations (8.20; 7.86), respectively. It is concluded that probiotic inclusion level had a significant effect on broiler growth responses, nutrient ADC, AME(n), and cecal microflora composition.
本研究旨在探究在肉鸡营养中添加 5 种细菌益生菌的水平对其的影响。525 只 1 日龄雄性科布肉鸡被分配到 5 个实验处理中，进行 6 周实验。实验处理接受玉米-豆粕无球虫药基础日粮，并根据添加情况分别标记为：无添加（C）、10(8)cfu 益生菌/千克日粮（P1）、10(9)cfu 益生菌/千克日粮（P2）、10(10)cfu 益生菌/千克日粮（P3）和 2.5 毫克维吉尼亚霉素/千克日粮（A）。每个处理有 3 个重复，每个重复 35 只肉鸡。测定了处理对肉鸡生长性能和生物标志物的影响，如回肠和全肠道养分消化率、血浆 Ig 浓度和盲肠微生物区系组成。当 P<0.05 时，认为处理间存在差异。与 P2（2163g）、C（2165g）和 P3（2167g）相比，P1（2293g）组的肉鸡总体体重增益显著更高，A（2230g）组居中且与 P1 组无差异。P1（1.80）和 A（1.80）组的饲料转化率均显著优于 P2（1.87）、C（1.89）和 P3（1.92）组。A 组的回肠表观消化率（ADC）和乙醚提取物更高。一般来说，A 和 P1 组的 DM、有机物、灰分、乙醚提取物和 AME(n)值的全肠道 ADC 更高。P1、C 和 P2 组的 CP 全肠道 ADC 更高。14 日龄和 42 日龄肉鸡的血浆 Ig 无处理差异。P2 和 P3 组可有效调节盲肠微生物区系组成。特别是，14 日龄和 42 日龄肉鸡的盲肠大肠埃希氏菌浓度（湿消化物的对数 cfu/g）分别为 P2（6.12）和 P3（4.90）最低，而 42 日龄时，P3 和 P2 组双歧杆菌（8.31；8.08）和乳酸杆菌（8.20；7.86）浓度最高。综上所述，益生菌添加水平对肉鸡生长反应、养分 ADC、AME(n)和盲肠微生物区系组成有显著影响。
