College of Animal Science and Technology, Zhejiang A & F University, Lin'an 311300, China.
Poult Sci. 2014 Jan;93(1):46-53. doi: 10.3382/ps.2013-03412.
This study was conducted to investigate the effects of Clostridium butyricumon growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88. Three hundred sixty 1-d-old broiler chickens were randomly divided into 4 treatments: negative control (NC) birds were fed a basal diet and not challenged with E. coli K88; positive control (PC) birds were fed a basal diet and challenged with E. coli K88; C. butyricum treatment (CB) birds were fed a diet containing 2 × 10(7) cfu C. butyricum/kg of diet and challenged with E. coli K88; and colistin sulfate treatment (CS) birds were fed a diet containing 20 mg of colistin sulfate/kg of diet and challenged with E. coli K88. Birds fed CB had greater (P < 0.05) BW than the PC birds from 3 to 21 d postchallenge. Birds fed CB had greater (P < 0.05) serum IgA and IgY at 14 d postchallenge, greater (P < 0.05) serum IgM at 21 d postchallenge, and greater (P < 0.05) mucosal secreted IgA at 3 and 7 d postchallenge than the PC birds. Birds fed CB had greater concentrations of serum complement component 3 at 14 d postchallenge, and greater (P < 0.05) concentrations of serum complement component 4 at 3, 7, and 14 d postchallenge than the PC birds. Birds in the CS or CB treatments had less cecal E. coli population at 3, 7, and 21 d postchallenge, and less cecal Clostridium perfringens counts at 21 d postchallenge compared with the PC birds. The CB treatment increased (P < 0.05) the population of cecal Lactobacillus at 3 d postchallenge and the number of cecal Bifidobacterium at 3, 14, and 21 d postchallenge in comparison with the PC treatment. The results indicate that dietary supplementation of CB promotes growth performance, improves immune function, and benefits the cecal microflora in Escherichia coli K88-challenged chickens.
本研究旨在探讨丁酸梭菌对感染大肠杆菌 K88 的肉鸡生长性能、免疫功能和盲肠微生物菌群的影响。将 360 只 1 日龄肉鸡随机分为 4 个处理组：阴性对照组（NC）鸡饲喂基础日粮，不攻毒大肠杆菌 K88；阳性对照组（PC）鸡饲喂基础日粮，攻毒大肠杆菌 K88；丁酸梭菌处理组（CB）鸡饲喂含 2×10(7)cfu 丁酸梭菌/kg 日粮，攻毒大肠杆菌 K88；硫酸粘菌素处理组（CS）鸡饲喂含 20mg/kg 硫酸粘菌素的日粮，攻毒大肠杆菌 K88。攻毒后 3 至 21 日，饲喂 CB 的鸡比 PC 鸡的 BW 更高（P＜0.05）。攻毒后 14 日，饲喂 CB 的鸡血清 IgA 和 IgY 更高（P＜0.05），攻毒后 21 日血清 IgM 更高（P＜0.05），攻毒后 3 和 7 日黏膜分泌型 IgA 更高（P＜0.05）；攻毒后 14 日，饲喂 CB 的鸡血清补体成分 3 浓度更高（P＜0.05），血清补体成分 4 浓度在攻毒后 3、7 和 14 日更高（P＜0.05）。与 PC 组相比，CS 或 CB 组鸡在攻毒后 3、7 和 21 日盲肠大肠杆菌数量更少，盲肠产气荚膜梭菌数量在攻毒后 21 日更少。与 PC 组相比，CB 组鸡在攻毒后 3 日盲肠乳酸杆菌数量更多，攻毒后 3、14 和 21 日盲肠双歧杆菌数量更多。结果表明，日粮中添加丁酸梭菌可促进大肠杆菌 K88 攻毒鸡的生长性能，提高免疫功能，有利于盲肠微生物菌群。
