El-Shinnawy Nashwa A, Abd-Elmageid Samira A, Alshailabi Eda M A
1Department of Zoology, Women's College for Arts, Science and Education, Ain Shams University, Cairo, Egypt.
Toxicol Ind Health. 2014 May;30(4):357-75. doi: 10.1177/0748233712457448. Epub 2012 Aug 22.
The present work is an attempt to elucidate the antiulcer activity of indole-3-carbinol (I3C), which is one of the anticarcinogenic phytochemicals found in the vegetables of Cruciferae family such as broccoli and cauliflower, alone or in combination with omeprazole (OMP), a proton pump inhibitor, to diminish the effects of induced acute gastric ulcer by aspirin (ASA) in male albino rats. A total of 48 adult male albino rats were used in the present study. Animals were divided into eight experimental groups (six animals each group). They were given different experimental inductions of ASA at a dose of 500 mg/kg/body weight, OMP at a dose of 20 mg/kg/body weight and I3C at a dose of 20 mg/kg/body weight either alone or in combination with each other orally for a duration of 7 days. Inner stomach features, ulcer index, pH activity, body weight, stomach weight, hematological investigations, serum total protein albumin and reduced glutathione activity were investigated in addition to the histological, histochemical and immunohistochemical stain of cyclooxygenase-2 to the stomach tissue of normal control, ulcerated and treated ulcerated rats. The results of this study revealed that oral administration of ASA to rats produced the expected characteristic mucosal lesions. OMP accelerated ulcer healing but the administration of I3C either alone or in combination with OMP to ASA-ulcerated rats produced a profound protection to the gastric mucosa from injury induced by ASA. Our results suggested that administration of antiulcer natural substances such as I3C in combination with the perused treatment such as OMP is a very important initiative in the development of new strategies in ulcer healing.
本研究旨在阐明吲哚 - 3 - 甲醇（I3C）的抗溃疡活性。I3C是十字花科蔬菜（如西兰花和花椰菜）中发现的抗癌植物化学物质之一，本研究观察其单独使用或与质子泵抑制剂奥美拉唑（OMP）联合使用时，对雄性白化大鼠阿司匹林（ASA）诱导的急性胃溃疡的影响。本研究共使用了48只成年雄性白化大鼠。动物被分为八个实验组（每组六只动物）。它们分别接受不同的实验诱导，给予剂量为500 mg/kg体重的ASA、剂量为20 mg/kg体重的OMP和剂量为20 mg/kg体重的I3C，单独或相互组合口服给药，持续7天。除了对正常对照、溃疡和治疗后的溃疡大鼠胃组织进行环氧化酶 - 2的组织学、组织化学和免疫组织化学染色外，还研究了胃内部特征、溃疡指数、pH活性、体重、胃重量、血液学检查、血清总蛋白白蛋白和还原型谷胱甘肽活性。本研究结果表明，给大鼠口服ASA会产生预期的典型黏膜损伤。OMP加速了溃疡愈合，但单独或与OMP联合给ASA溃疡大鼠施用I3C可对胃黏膜提供显著保护，使其免受ASA诱导的损伤。我们的结果表明，将抗溃疡天然物质（如I3C）与常用治疗药物（如OMP）联合使用，是开发溃疡愈合新策略的一项非常重要的举措。
