McHedlishvili Levan, Mazurov Vladimir, Tanaka Elly M
DFG-Center for Regenerative Therapies Dresden, Cluster of Excellence, University of Technology Dresden, Dresden, Germany.
Methods Mol Biol. 2012;916:197-202. doi: 10.1007/978-1-61779-980-8_15.
Urodele amphibians such as axolotl are well known for their regenerative potential of the damaged central nervous system structures. Upon tail amputation, neural stem cells behind the amputation plane undergo self-renewing divisions and contribute to the functional spinal cord in the newly formed regenerate. The neural stem cells, harboring this potential, can be isolated from the animal and cultured under the suspension conditions. After 2-3 weeks in vitro they will proliferate and form the floating aggregates of the spherical shape, so-called neurospheres. Reimplanted back into the animal, the neurospheres can efficiently integrate in the spinal cord lesion and contribute to the following spinal cord regeneration events. Here we demonstrate the unique method of the axolotl tail spinal cord regeneration from the implanted neurosphere.
诸如蝾螈之类的有尾两栖动物以其受损中枢神经系统结构的再生潜力而闻名。在尾巴被截断后，截断平面后方的神经干细胞会进行自我更新分裂，并为新形成的再生组织中的功能性脊髓做出贡献。具有这种潜力的神经干细胞可以从动物体内分离出来，并在悬浮条件下进行培养。在体外培养2至3周后，它们会增殖并形成球形的漂浮聚集体，即所谓的神经球。将神经球重新植入动物体内后，它们可以有效地整合到脊髓损伤部位，并促进后续的脊髓再生过程。在这里，我们展示了从植入的神经球实现蝾螈尾巴脊髓再生的独特方法。
