Thygesen Mathias Møller, Rasmussen Mikkel Mylius, Madsen Jesper Guldsmed, Pedersen Michael, Lauridsen Henrik
Comparative Medicine Laboratory Department of Clinical Medicine, Aarhus University Palle Juul-Jensens Boulevard 998200 Aarhus N Denmark.
Department of Neurosurgery Aarhus University Hospital Noerrebrogade 448000 Aarhus C Denmark.
Regeneration (Oxf). 2017 Jul 27;4(3):124-131. doi: 10.1002/reg2.80. eCollection 2017 Jun.
The Mexican axolotl () is an important model species in regenerative biology. Traditionally, axolotls are anesthetized using benzocaine or MS-222, both of which act to inhibit voltage gated sodium channels thereby preventing action potential propagation. In some neurophysiological experiments this is not desirable; therefore we tested propofol as an alternative anesthetic in the axolotl. We evaluated benzocaine, MS-222, and propofol's cardiovascular effects, effects on action potential propagation in the spinal cord, and gross limb regenerative effects. We found that propofol is applicable as a general anesthetic in the axolotl allowing for neurophysiological experiments and yielding a stable anesthesia with significantly less cardiovascular effect than both benzocaine and MS-222. Additionally, propofol did not affect gross limb regeneration. In conclusion we suggest the consideration of propofol as an alternative immersion anesthetic to benzocaine and MS-222.
墨西哥钝口螈（）是再生生物学中的一种重要模式物种。传统上，钝口螈使用苯佐卡因或MS - 222进行麻醉，这两种药物都通过抑制电压门控钠通道来阻止动作电位的传播。在一些神经生理学实验中，这是不理想的；因此，我们测试了丙泊酚作为钝口螈的替代麻醉剂。我们评估了苯佐卡因、MS - 222和丙泊酚对心血管的影响、对脊髓动作电位传播的影响以及对肢体大体再生的影响。我们发现丙泊酚可作为钝口螈的全身麻醉剂，适用于神经生理学实验，并且能产生稳定的麻醉效果，其对心血管的影响明显小于苯佐卡因和MS - 222。此外，丙泊酚不影响肢体大体再生。总之，我们建议考虑将丙泊酚作为苯佐卡因和MS - 222的替代浸泡麻醉剂。
