Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Chelmonskiego 37/41, 51-630 Wroclaw, Poland.
Amino Acids. 2013 Feb;44(2):315-20. doi: 10.1007/s00726-012-1379-7. Epub 2012 Aug 23.
Numerous studies have shown that food proteins may be a source of bioactive peptides. Those peptides are encrypted in the protein sequence. They stay inactive within the parental protein until release by proteolytic enzymes (Mine and Kovacs-Nolan in Worlds Poult Sci J 62(1):87-95, 2006; Hartman and Miesel in Curr Opin Biotechnol 18:163-169, 2007). Once released the bioactive peptides exhibit several biofunctionalities and may serve therapeutic roles in body systems. Opioid peptides, peptides lowering high blood pressure, inhibiting platelet aggregation as well as being carriers of metal ions and peptides with immunostimulatory, antimicrobial and antioxidant activities have been described (Hartman and Miesel in Curr Opin Biotechnol 18:163-169, 2007). The biofunctional abilities of the peptides have therefore aroused a lot of scientific, technological and consumer interest with respect to the role of dietary proteins in controlling and influencing health (Möller et al. in Eur J Nutr 47(4):171-182, 2008). Biopeptides may find wide application in food production, the cosmetics industry as well as in the prevention and treatment of various medical conditions. They are manufactured by chemical and biotechnological methods (Marx in Chem Eng News 83(11):17-24. 2005; Hancock and Sahl in Nat Biotechnol 24(12):1551-1557, 2006). Depending on specific needs (food or pharmaceutical industry) different degrees of peptide purifications are required. This paper discusses the practicability of manufacturing bioactive peptides, especially from food proteins.
大量研究表明，食物蛋白可能是生物活性肽的来源。这些肽被加密在蛋白质序列中。在蛋白酶（Mine 和 Kovacs-Nolan 在《世界家禽科学杂志》62（1）：87-95，2006；Hartman 和 Miesel 在《当代生物技术观点》18：163-169，2007）的作用下，它们才从母体蛋白中释放出来并具有生物活性。这些生物活性肽一旦被释放出来，就具有多种生物功能，并可能在身体系统中发挥治疗作用。已经描述了阿片样肽、降低高血压的肽、抑制血小板聚集的肽以及作为金属离子载体和具有免疫刺激、抗菌和抗氧化活性的肽（Hartman 和 Miesel 在《当代生物技术观点》18：163-169，2007）。这些肽的生物功能能力引起了科学界、技术界和消费者的极大兴趣，因为它们关注的是膳食蛋白在控制和影响健康方面的作用（Möller 等人在《欧洲营养杂志》47（4）：171-182，2008）。生物活性肽在食品生产、化妆品行业以及各种医疗条件的预防和治疗中可能有广泛的应用。它们可以通过化学和生物技术方法制造（Marx 在《化学工程新闻》83（11）：17-24，2005；Hancock 和 Sahl 在《自然生物技术》24（12）：1551-1557，2006）。根据特定需求（食品或制药行业），需要对肽进行不同程度的纯化。本文讨论了制造生物活性肽的实用性，特别是从食物蛋白中制造生物活性肽。
