玻璃化对人中期 II 卵母细胞线粒体膜电位的影响。
Effect of vitrification on mitochondrial membrane potential in human metaphase II oocytes.
机构信息
Chongqing Medical University, 1 Yi Xue Yuan ST, Yu Zhong Dis, Chongqing 400016, China.
出版信息
J Assist Reprod Genet. 2012 Oct;29(10):1045-50. doi: 10.1007/s10815-012-9848-1. Epub 2012 Aug 23.
Abstract
OBJECTIVE
The aim of this study was to evaluate the impact of vitrification on mitochondrial membrane potential (ΔΨm) in human metaphase II (MII) oocytes, and the changes of ΔΨm on thawed MII oocytes.
METHODS
MII oocytes were obtained from clinical IVF cycles when the oocytes were failed to fertilization within 24 h after insemination. All oocytes were randomly divided into 4 groups: non-frozen (fresh group), cultured for 0 h (0 h group), 2 h (2 h group) and 4 h (4 h group) after vitrification/thawing. All oocytes were stained with the ΔΨm-specific probe JC-1 and detected by laser scanning confocal microscope (LSCM) for mitochondrial analysis.
RESULTS
The ΔΨm of oocytes was significantly decreased in 0 h and 2 h groups when compared with fresh group (0.93, 1.09 vs 1.34, P < 0.05), but similar between 4 h group and fresh group (1.30 vs 1.34, P > 0.05).
CONCLUSION
In the vitrification/thawing process, the ΔΨm of MII oocytes could have temporally dynamic changes within 2 h after thawing but would be fully recovered after 4 h culture.
摘要
目的
本研究旨在评估玻璃化对人中期 II（MII）卵母细胞线粒体膜电位（ΔΨm）的影响，以及ΔΨm 在解冻 MII 卵母细胞中的变化。
方法
从临床 IVF 周期中获得 MII 卵母细胞，这些卵母细胞在受精后 24 小时内未能受精。所有卵母细胞随机分为 4 组：未冷冻（新鲜组）、玻璃化/解冻后培养 0 小时（0 小时组）、2 小时（2 小时组）和 4 小时（4 小时组）。所有卵母细胞均用 ΔΨm 特异性探针 JC-1 染色，并通过激光扫描共聚焦显微镜（LSCM）进行线粒体分析。
结果
与新鲜组相比，0 小时和 2 小时组的卵母细胞ΔΨm 显著降低（0.93、1.09 与 1.34，P<0.05），但 4 小时组与新鲜组之间无差异（1.30 与 1.34，P>0.05）。
结论
在玻璃化/解冻过程中，解冻后 2 小时内 MII 卵母细胞的ΔΨm 可能会发生暂时的动态变化，但在 4 小时培养后会完全恢复。
相似文献
1
Effect of vitrification on mitochondrial membrane potential in human metaphase II oocytes.玻璃化对人中期 II 卵母细胞线粒体膜电位的影响。
J Assist Reprod Genet. 2012 Oct;29(10):1045-50. doi: 10.1007/s10815-012-9848-1. Epub 2012 Aug 23.
2
Cryopreservation of metaphase II human oocytes effects mitochondrial membrane potential: implications for developmental competence.中期II期人类卵母细胞的冷冻保存影响线粒体膜电位：对发育能力的影响
Hum Reprod. 2004 Aug;19(8):1861-6. doi: 10.1093/humrep/deh313. Epub 2004 Jun 30.
3
Impact of vitrification on the mitochondrial activity and redox homeostasis of human oocyte.玻璃化对人卵母细胞线粒体活性和氧化还原稳态的影响。
Hum Reprod. 2016 Aug;31(8):1850-8. doi: 10.1093/humrep/dew130. Epub 2016 May 31.
4
Improved cryotolerance and developmental potential of in vitro and in vivo matured mouse oocytes by supplementing with a glutathione donor prior to vitrification.在玻璃化之前补充谷胱甘肽供体可提高体外和体内成熟小鼠卵母细胞的耐冻性和发育潜力。
Mol Hum Reprod. 2016 Dec;22(12):867-881. doi: 10.1093/molehr/gaw059. Epub 2016 Sep 7.
5
Slow freezing and vitrification differentially modify the gene expression profile of human metaphase II oocytes.慢速冷冻和玻璃化对人中期 II 卵母细胞的基因表达谱有不同的影响。
Hum Reprod. 2012 Jul;27(7):2160-8. doi: 10.1093/humrep/des153. Epub 2012 May 15.
6
Effects of MICU1-Mediated Mitochondrial Calcium Uptake on Energy Metabolism and Quality of Vitrified-Thawed Mouse Metaphase II Oocytes.MICU1 介导线粒体钙摄取对玻璃化冷冻解冻小鼠中期 II 卵母细胞能量代谢和质量的影响。
Int J Mol Sci. 2022 Aug 3;23(15):8629. doi: 10.3390/ijms23158629.
7
Changes in mitochondrial function in porcine vitrified MII-stage oocytes and their impacts on apoptosis and developmental ability.猪玻璃化MII期卵母细胞线粒体功能的变化及其对细胞凋亡和发育能力的影响。
Cryobiology. 2015 Oct;71(2):291-8. doi: 10.1016/j.cryobiol.2015.08.002. Epub 2015 Aug 3.
8
Effect of mouse oocyte vitrification on mitochondrial membrane potential and distribution.小鼠卵母细胞玻璃化冷冻对线粒体膜电位及分布的影响
J Huazhong Univ Sci Technolog Med Sci. 2014 Feb;34(1):99-102. doi: 10.1007/s11596-014-1238-8. Epub 2014 Feb 6.
9
Cytokeratin distribution and expression during the maturation of mouse germinal vesicle oocytes after vitrification.玻璃化冷冻后小鼠生发泡期卵母细胞成熟过程中细胞角蛋白的分布和表达。
Cryobiology. 2013 Jun;66(3):261-6. doi: 10.1016/j.cryobiol.2013.02.062. Epub 2013 Mar 7.
10
In-vitro maturation of human oocytes: before or after vitrification?人卵的体外成熟：玻璃化之前还是之后？
J Assist Reprod Genet. 2012 Jun;29(6):507-12. doi: 10.1007/s10815-012-9751-9. Epub 2012 Apr 4.
引用本文的文献
1
Maternal total sleep deprivation causes oxidative stress and mitochondrial dysfunction in oocytes associated with fertility decline in mice.母体总睡眠剥夺导致卵子氧化应激和线粒体功能障碍，与小鼠生育能力下降有关。
PLoS One. 2024 Oct 16;19(10):e0306152. doi: 10.1371/journal.pone.0306152. eCollection 2024.
2
Cytokine-Supplemented Maturation Medium Enhances Cytoplasmic and Nuclear Maturation in Bovine Oocytes.细胞因子补充成熟培养基可增强牛卵母细胞的细胞质和细胞核成熟。
Animals (Basel). 2024 Jun 20;14(12):1837. doi: 10.3390/ani14121837.
3
Ultra-Fast Vitrification: Minimizing the Toxicity of Cryoprotective Agents and Osmotic Stress in Mouse Oocyte Cryopreservation.超快速玻璃化冷冻法：在小鼠卵母细胞冷冻保存中减少细胞毒性保护剂和渗透应激的影响。
Int J Mol Sci. 2024 Feb 4;25(3):1884. doi: 10.3390/ijms25031884.
4
Ovarian tissue cryopreservation and transplantation: a review on reactive oxygen species generation and antioxidant therapy.卵巢组织冷冻保存与移植：活性氧生成及抗氧化治疗综述
Cell Tissue Res. 2023 Sep;393(3):401-423. doi: 10.1007/s00441-023-03794-2. Epub 2023 Jun 17.
5
Microencapsulation and nanowarming enables vitrification cryopreservation of mouse preantral follicles.微囊化和纳米加热使小鼠原始卵泡的玻璃化冷冻保存成为可能。
Nat Commun. 2022 Dec 15;13(1):7515. doi: 10.1038/s41467-022-34549-2.
6
Assessment of Mitochondrial Function and Developmental Potential of Mouse Oocytes after Mitoquinone Supplementation during Vitrification.线粒体醌干预玻璃化冷冻对小鼠卵母细胞线粒体功能和发育潜能的评估。
J Am Assoc Lab Anim Sci. 2021 Jul 1;60(4):388-395. doi: 10.30802/AALAS-JAALAS-20-000123. Epub 2021 May 12.
7
Mitochondrial Dysfunction and Oxidative Stress Caused by Cryopreservation in Reproductive Cells.冷冻保存导致生殖细胞中的线粒体功能障碍和氧化应激。
Antioxidants (Basel). 2021 Feb 24;10(3):337. doi: 10.3390/antiox10030337.
8
Blastocyst mitochondrial DNA (mtDNA) is not affected by oocyte vitrification: a sibling oocyte study.囊胚线粒体DNA（mtDNA）不受卵母细胞玻璃化冷冻的影响：一项同胞卵母细胞研究。
J Assist Reprod Genet. 2020 Jun;37(6):1387-1397. doi: 10.1007/s10815-020-01795-6. Epub 2020 May 6.
9
Preincubation with glutathione ethyl ester improves the developmental competence of vitrified mouse oocytes.谷胱甘肽乙酯预孵育可提高玻璃化冷冻小鼠卵母细胞的发育能力。
J Assist Reprod Genet. 2018 Jul;35(7):1169-1178. doi: 10.1007/s10815-018-1215-4. Epub 2018 Jun 6.
10
Vitrification of human immature oocytes before and after in vitro maturation: a review.人类未成熟卵母细胞玻璃化冷冻保存：综述。
J Assist Reprod Genet. 2017 Nov;34(11):1413-1426. doi: 10.1007/s10815-017-1005-4. Epub 2017 Aug 18.
本文引用的文献
1
High developmental rates of mouse oocytes cryopreserved by an optimized vitrification protocol: the effects of cryoprotectants, calcium and cumulus cells.通过优化玻璃化方案冷冻保存的小鼠卵母细胞具有较高的发育率：冷冻保护剂、钙和卵丘细胞的影响。
J Reprod Dev. 2011 Dec;57(6):675-80. doi: 10.1262/jrd.11-066h. Epub 2011 Jul 22.
2
Clinical application of oocyte vitrification: a systematic review and meta-analysis of randomized controlled trials.卵母细胞玻璃化冷冻的临床应用：随机对照试验的系统评价和荟萃分析。
Fertil Steril. 2011 Aug;96(2):277-85. doi: 10.1016/j.fertnstert.2011.06.030. Epub 2011 Jun 30.
3
Comparison outcome of fresh and vitrified donor oocytes in an egg-sharing donation program.新鲜和玻璃化捐赠卵母细胞在卵子分享捐赠计划中的比较结果。
Fertil Steril. 2011 May;95(6):1996-2000. doi: 10.1016/j.fertnstert.2011.02.035. Epub 2011 Mar 15.
4
Experience of freezing human oocytes using sodium-depleted media.使用去钠培养基冷冻人卵母细胞的经验。
Reprod Biomed Online. 2011 Jan;22(1):83-7. doi: 10.1016/j.rbmo.2010.09.010. Epub 2010 Nov 30.
5
Effect of vitrification and thawing on human oocyte ATP concentration.玻璃化冷冻和解冻对人卵母细胞 ATP 浓度的影响。
Fertil Steril. 2011 Apr;95(5):1839-41. doi: 10.1016/j.fertnstert.2010.10.040. Epub 2010 Nov 11.
6
Qualitative and morphometric analysis of the ultrastructure of human oocytes cryopreserved by two alternative slow cooling protocols.两种不同的慢速冷冻方案对人卵母细胞超微结构的定性和形态计量分析。
J Assist Reprod Genet. 2010 Apr;27(4):131-40. doi: 10.1007/s10815-010-9394-7. Epub 2010 Feb 23.
7
Ultrastructural markers of quality in human mature oocytes vitrified using cryoleaf and cryoloop.使用 cryoleaf 和 cryoloop 玻璃化冷冻的人类成熟卵母细胞的超微结构质量标志物。
Reprod Biomed Online. 2009;19 Suppl 3:17-27. doi: 10.1016/s1472-6483(10)60280-5.
8
IVF/ICSI outcomes between cycles with luteal estradiol (E2) pre-treatment before GnRH antagonist protocol and standard long GnRH agonist protocol: a prospective and randomized study.在 GnRH 拮抗剂方案前采用黄体期雌二醇（E2）预处理的周期与标准长效 GnRH 激动剂方案的周期之间的体外受精/卵胞浆内单精子注射结局：一项前瞻性随机研究。
J Assist Reprod Genet. 2009 Mar;26(2-3):105-11. doi: 10.1007/s10815-009-9300-3. Epub 2009 Feb 19.
9
Slow cooling of human oocytes: ultrastructural injuries and apoptotic status.人类卵母细胞的缓慢冷却：超微结构损伤与凋亡状态
Fertil Steril. 2009 Apr;91(4):1023-34. doi: 10.1016/j.fertnstert.2008.01.076. Epub 2008 Mar 25.
10
Truths and myths of oocyte sensitivity to controlled rate freezing.
Reprod Biomed Online. 2007 Jul;15(1):24-30. doi: 10.1016/s1472-6483(10)60687-6.
Zotero 插件