Center of Molecular Immunology, Havana, Cuba.
Biotechnol Prog. 2012 Nov-Dec;28(6):1491-8. doi: 10.1002/btpr.1618. Epub 2012 Oct 18.
In the present work, the main fluid flow features inside a rotating cylindrical filtration (RCF) system used as external cell retention device for animal cell perfusion processes were investigated using particle image velocimetry (PIV). The motivation behind this work was to provide experimental fluid dynamic data for such turbulent flow using a high-permeability filter, given the lack of information about this system in the literature. The results shown herein gave evidence that, at the boundary between the filter mesh and the fluid, a slip velocity condition in the tangential direction does exist, which had not been reported in the literature so far. In the RCF system tested, this accounted for a fluid velocity 10% lower than that of the filter tip, which could be important for the cake formation kinetics during filtration. Evidence confirming the existence of Taylor vortices under conditions of turbulent flow and high permeability, typical of animal cell perfusion RCF systems, was obtained. Second-order turbulence statistics were successfully calculated. The radial behavior of the second-order turbulent moments revealed that turbulence in this system is highly anisotropic, which is relevant for performing numerical simulations of this system.
在本工作中，使用粒子图像测速法（PIV）研究了作为动物细胞灌注过程外部细胞保留装置的旋转圆柱过滤（RCF）系统内的主要流体流动特征。这项工作的动机是提供这种使用高通量过滤器的湍流流动的实验流体动力学数据，因为迄今为止文献中缺乏有关该系统的信息。本文所示的结果表明，在过滤网格和流体之间的边界处，确实存在切向方向的滑移速度条件，这迄今为止在文献中尚未报道过。在所测试的 RCF 系统中，这导致过滤尖端的流体速度降低了 10%，这对于过滤过程中的滤饼形成动力学可能很重要。获得了在典型的动物细胞灌注 RCF 系统中存在湍流和高通量条件下的泰勒涡旋的证据。成功计算了二阶湍流统计数据。二阶湍流矩的径向行为表明，该系统中的湍流具有很强的各向异性，这对于对该系统进行数值模拟很重要。
