Pazla Roni, Yanti Gusri, Jamarun Novirman, Zain Mardiati, Triani Hera Dwi, Putri Ezi Masdia, Srifani Anifah
Department of Animal Nutrition and Feed Technology, Faculty of Animal Science, Universitas Andalas, Limau Manis, Padang 25163, Indonesia.
Department of Agricultural Extension, Faculty of Social, Science and Education, Prima Nusantara Bukittinggi University, Bukittinggi 26122, Indonesia.
Saudi J Biol Sci. 2024 Jul;31(7):104006. doi: 10.1016/j.sjbs.2024.104006. Epub 2024 May 8.
Phytate content in feed ingredients can negatively impact digestibility and palatability. To address this issue, it is necessary to study microbes capable of breaking down phytate content. This study aimed to isolate and characterize phytase-producing bacteria from decaying materials rich in phytic acid. The research was conducted in several stages. The first stage involved isolating phytase-producing bacteria from the acidification of using growth media containing Na-phytate. Bacterial isolates that produced clear zones were then tested for their activity and ability to produce several enzymes, specifically phytase, cellulase, and protease. The next step was to test the morphological characteristics of the bacterial isolate. The final stage of bacterial identification consisted of DNA isolation, followed by PCR amplification of the 16S rRNA gene, DNA sequence homology analysis, and construction of a phylogenetic tree. Based on research, three isolates were found to produce clear phytase zones: isolates R5 (20.3 mm), R7 (16.1 mm) and R8 (31.7 mm). All isolates were able to produce the enzymes phytase (5.45-6.54 U/ml), cellulase (2.60-2.92 U/ml), and protease (22.2-23.4 U/ml). Metagenomic testing identified isolate R7 and R8 as and isolate R5 as . The isolation and characterization of phytase-producing bacteria from acidification resulted in the identification of two promising candidates that can be applied as sources of phytase producers. Phytase-producing bacteria can be utilized to improve digestibility and palatability in animal feed.
饲料原料中的植酸盐含量会对消化率和适口性产生负面影响。为解决这一问题，有必要研究能够分解植酸盐含量的微生物。本研究旨在从富含植酸的腐烂物质中分离和鉴定产植酸酶的细菌。该研究分几个阶段进行。第一阶段是使用含植酸钠的生长培养基，通过酸化作用分离产植酸酶的细菌。然后对产生透明圈的细菌分离株进行活性测试以及几种酶（特别是植酸酶、纤维素酶和蛋白酶）产生能力的测试。下一步是测试细菌分离株的形态特征。细菌鉴定的最后阶段包括DNA分离，随后进行16S rRNA基因的PCR扩增、DNA序列同源性分析以及系统发育树的构建。基于研究，发现三个分离株产生明显的植酸酶透明圈：分离株R5（20.3毫米）、R7（16.1毫米）和R8（31.7毫米）。所有分离株都能够产生植酸酶（5.45 - 6.54 U/ml）、纤维素酶（2.60 - 2.92 U/ml）和蛋白酶（22.2 - 23.4 U/ml）。宏基因组测试确定分离株R7和R8为 ，分离株R5为 。从酸化作用中分离和鉴定产植酸酶的细菌，得到了两个有前景的候选菌株，可作为植酸酶产生菌的来源。产植酸酶的细菌可用于提高动物饲料的消化率和适口性。
