Ku Gregory M, Kim Hail, Vaughn Ian W, Hangauer Matthew J, Myung Oh Chang, German Michael S, McManus Michael T
513 Parnassus Avenue, Box 0534, San Francisco, California 94143-0534, USA.
Mol Endocrinol. 2012 Oct;26(10):1783-92. doi: 10.1210/me.2012-1176. Epub 2012 Aug 21.
The pancreatic β-cell is critical for the maintenance of glycemic control. Knowing the compendium of genes expressed in β-cells will further our understanding of this critical cell type and may allow the identification of future antidiabetes drug targets. Here, we report the use of next-generation sequencing to obtain nearly 1 billion reads from the polyadenylated RNA of islets and purified β-cells from mice. These data reveal novel examples of β-cell-specific splicing events, promoter usage, and over 1000 long intergenic noncoding RNA expressed in mouse β-cells. Many of these long intergenic noncoding RNA are β-cell specific, and we hypothesize that this large set of novel RNA may play important roles in β-cell function. Our data demonstrate unique features of the β-cell transcriptome.
胰腺β细胞对于维持血糖控制至关重要。了解β细胞中表达的基因全集将加深我们对这种关键细胞类型的理解，并可能有助于识别未来的抗糖尿病药物靶点。在此，我们报告使用下一代测序技术从小鼠胰岛和纯化的β细胞的聚腺苷酸化RNA中获得了近10亿条读数。这些数据揭示了β细胞特异性剪接事件、启动子使用的新例子，以及在小鼠β细胞中表达的1000多种长链基因间非编码RNA。这些长链基因间非编码RNA中的许多都是β细胞特异性的，我们推测这一大组新的RNA可能在β细胞功能中发挥重要作用。我们的数据证明了β细胞转录组的独特特征。
