1. Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77005, USA;
Theranostics. 2012;2(8):777-87. doi: 10.7150/thno.4494. Epub 2012 Aug 9.
MUC1 is a large, heavily glycosylated transmembrane glycoprotein that is proposed to create a protective microenvironment in many adenocarcinomas. Here we compare MUC1 and the well studied cell surface receptor target, EGFR, as gold nanoparticle (AuNP) targets and their subsequent vapor nanobubble generation efficacy in the human epithelial cell line, HES. Although EGFR and MUC1 were both highly expressed in these cells, TEM and confocal images revealed MUC1 as a superior target for nanoparticle intracellular accumulation and clustering. The MUC1-targeted AuNP intracellular clusters also generated significantly larger vapor nanobubbles. Our results demonstrate the promising opportunities MUC1 offers to improve the efficacy of targeted nanoparticle based approaches.
黏蛋白 1（MUC1）是一种大型的、高度糖基化的跨膜糖蛋白，据推测它可以在许多腺癌中形成保护性的微环境。在这里，我们将 MUC1 与研究充分的细胞表面受体靶标 EGFR 进行比较，评估它们作为金纳米颗粒（AuNP）靶标的效果，以及在人上皮细胞系 HES 中随后生成蒸汽纳米气泡的效果。尽管 EGFR 和 MUC1 在这些细胞中都高度表达，但 TEM 和共聚焦图像显示，MUC1 是纳米颗粒细胞内积累和聚集的更好靶标。MUC1 靶向的 AuNP 细胞内聚集体也产生了明显更大的蒸汽纳米气泡。我们的结果表明，MUC1 为提高基于靶向纳米颗粒的方法的疗效提供了有前途的机会。
