Kubbies M, Schindler D, Hoehn H, Rabinovitch P S
J Cell Physiol. 1985 Nov;125(2):229-34. doi: 10.1002/jcp.1041250209.
An improved BrdU-Hoechst flow assay was applied to cell kinetic studies of human lymphocyte cultures during a 24-96 hr interval after PHA stimulation. The assay shows that the duration of the initial lag phase and the proportions of noncycling cells increase as a function of donor age, whereas the rates of transition from each cell cycle compartment to the next decrease. Cell cycle arrest occurs in the first S and G2 phase after stimulation of lymphocytes from a 75-year-old donor but not from younger donors. The data are consistent with several models of cell cycle kinetics, so long as these models are modified to include a fraction of noncycling cells in each cell cycle compartment.
一种改进的BrdU-Hoechst流式细胞术检测方法被应用于PHA刺激后24 - 96小时内人淋巴细胞培养物的细胞动力学研究。该检测方法表明，初始延迟期的持续时间和非循环细胞的比例随供体年龄的增加而增加，而从每个细胞周期阶段向下一个阶段的转变速率则降低。来自75岁供体的淋巴细胞受到刺激后，细胞周期停滞发生在第一个S期和G2期，而年轻供体的淋巴细胞则不会。只要这些模型经过修改，在每个细胞周期阶段纳入一部分非循环细胞，这些数据就与几种细胞周期动力学模型一致。
