Department of Cardiology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, Shanghai, 200011, China.
Dig Dis Sci. 2013 Feb;58(2):423-30. doi: 10.1007/s10620-012-2356-4. Epub 2012 Aug 24.
Abnormal expression of early growth response gene 1 (Egr-1) and β-catenin may play a crucial role in the development and progression of human cancer. However, little is known about the expression and underlying molecular mechanisms in which Egr-1 and β-catenin are involved in the development and progression of gastric cancer.
The purpose of this study was to elucidate the potential relationship between Egr-1 and β-catenin expression in gastric cancer, which contributes to finding new molecular carcinogenesis as a potential therapeutic target for gastric cancer.
In a sample of 102 cases of human gastric cancer, the expression of Egr-1 and β-catenin was detected using immunohistochemistry. Egr-1 gene was transfected into gastric cancer SGC7901 cells and its role in proliferation and cell invasion was detected by MTT assay, flow cytometry, wound-healing and transwell invasion assay. Western blot analysis was used to study the expression of β-catenin and cyclin D1 proteins.
Upregulated Egr-1 and β-catenin protein expression were strongly correlated with cancer progression and depth of invasion in gastric cancer. β-catenin, present mainly in cytoplasmic and nucleus of gastric cancer cells, was also positively correlated with Egr-1 expression in gastric cancer. Furthermore, the overexpression of Egr-1 upregulated β-catenin expression level, promoted cell proliferation, increased cell population in S-phase and enhanced gastric cancer cell migration and invasion in vitro.
Egr-1 might contribute to gastric cancer proliferation and invasion through activation of the β-catenin signaling pathway.
早期生长反应基因 1（Egr-1）和β-连环蛋白的异常表达可能在人类癌症的发生和发展中起关键作用。然而，Egr-1 和β-连环蛋白在胃癌的发生和发展中表达及其潜在的分子机制知之甚少。
本研究旨在阐明胃癌中 Egr-1 和β-连环蛋白表达之间的潜在关系，以期发现新的分子致癌机制，为胃癌的潜在治疗靶点提供依据。
采用免疫组织化学法检测 102 例人胃癌组织中 Egr-1 和β-连环蛋白的表达。用 Egr-1 基因转染胃癌 SGC7901 细胞，MTT 法、流式细胞术、划痕愈合实验和 Transwell 侵袭实验检测 Egr-1 对细胞增殖和侵袭的影响，Western blot 分析检测β-连环蛋白和细胞周期蛋白 D1 蛋白的表达。
Egr-1 和β-连环蛋白蛋白表达上调与胃癌的进展和浸润深度密切相关。β-连环蛋白主要存在于胃癌细胞的细胞质和细胞核中，在胃癌中与 Egr-1 的表达呈正相关。此外，Egr-1 的过表达上调了β-连环蛋白的表达水平，促进了细胞增殖，增加了 S 期细胞群，并增强了胃癌细胞的体外迁移和侵袭能力。
Egr-1 可能通过激活β-连环蛋白信号通路促进胃癌的增殖和侵袭。
