LncRNA SUMO1P3 通过 Wnt/β-catenin 信号通路调节胃癌细胞的侵袭、迁移和细胞周期。

LncRNA SUMO1P3 regulates the invasion, migration and cell cycle of gastric cancer cells through Wnt/β-catenin signaling pathway.

机构信息

Department of Gastroenterology, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian, China.

Department of Gynaecology, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian, China.

出版信息

J Recept Signal Transduct Res. 2021 Dec;41(6):574-581. doi: 10.1080/10799893.2020.1836494. Epub 2020 Nov 12.

DOI:10.1080/10799893.2020.1836494

PMID:33179980

Abstract

OBJECTIVE

To investigate the regulatory effect of long non-coding RNA (lncRNA) SUMO1P3 on invasion, migration and cell cycle of gastric cancer (GC) cells through Wnt/β-catenin signaling pathway.

METHODS

Tumor tissues and adjacent normal tissues from the GC patients were collected, and human normal gastric epithelial cells GES1 and GC cells SGC-7901, MKN45, HGC-27 and AGS were selected for study. The expression of SUMO1P3 in GC tissues and cells were detected by RT-qPCR. The effects of SUMO1P3 on the proliferation, invasion and migration of SGC-7901 and MKN45 cells were detected by CCK-8, transwell and wound healing assay respectively, and the effects of SUMO1P3 on apoptosis and cycle progression of SGC-7901 and MKN45 cells were detected by flow cytometry. The expressions of Wnt/β-catenin pathway-related and cell cycle-related proteins were detected by Western blot.

RESULTS

The expression of SUMO1P3 was significantly upregulated in GC tissues and cell lines. Downregulation of SUMO1P3 significantly inhibited the SGC-7901 and MKN45 cell proliferation, invasion, migration, and cycle progression and promoted the cell apoptosis, while overexpression of SUMO1P3 showed the opposite effect. Further study showed that downregulation of SUMO1P3 significantly reduced the expressions of Wnt1, β-catenin, c-myc, and Cyclin D1 in SGC-7901 and MKN45 cells.

CONCLUSION

SUMO1P3 may promote invasion, migration, and cycle progression of SGC-7901 and MKN45 cells by enhancing the Wnt/β-catenin pathway.

摘要

目的

通过 Wnt/β-catenin 信号通路研究长链非编码 RNA（lncRNA）SUMO1P3 对胃癌（GC）细胞侵袭、迁移和细胞周期的调控作用。

方法

收集胃癌患者的肿瘤组织和相邻正常组织，选择人正常胃上皮细胞 GES1 和 GC 细胞 SGC-7901、MKN45、HGC-27 和 AGS 进行研究。采用 RT-qPCR 检测 GC 组织和细胞中 SUMO1P3 的表达。CCK-8、Transwell 和划痕愈合实验分别检测 SUMO1P3 对 SGC-7901 和 MKN45 细胞增殖、侵袭和迁移的影响，流式细胞术检测 SUMO1P3 对 SGC-7901 和 MKN45 细胞凋亡和周期进程的影响。Western blot 检测 Wnt/β-catenin 通路相关和细胞周期相关蛋白的表达。

结果

SUMO1P3 在 GC 组织和细胞系中表达明显上调。SUMO1P3 下调显著抑制 SGC-7901 和 MKN45 细胞增殖、侵袭、迁移和细胞周期进程，促进细胞凋亡，而过表达 SUMO1P3 则表现出相反的效果。进一步研究表明，SUMO1P3 下调显著降低了 SGC-7901 和 MKN45 细胞中 Wnt1、β-catenin、c-myc 和 Cyclin D1 的表达。