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一种用于宏基因组筛选和纯培养物中外源微生物蛋白酶活性检测的稳健平板检测法。

A robust plate assay for detection of extracellular microbial protease activity in metagenomic screens and pure cultures.

机构信息

Cardiff School of Biosciences, Cardiff University, Cardiff, CF10 3AT, UK.

出版信息

J Microbiol Methods. 2012 Oct;91(1):144-6. doi: 10.1016/j.mimet.2012.08.006. Epub 2012 Aug 19.

DOI:10.1016/j.mimet.2012.08.006
PMID:22921428
Abstract

A robust, efficient and cost-effective agar that utilises lactose free milk powder for identification of bacterial protease activity in pure cultures and metagenomic screens has been developed and tested on protease positive bacteria, selected strains and false protease positives isolated from a previously constructed metagenomic library.

摘要

已经开发并测试了一种稳健、高效且具有成本效益的琼脂，该琼脂利用无乳糖奶粉来鉴定纯培养物和宏基因组筛选中的细菌蛋白酶活性。该琼脂已在先前构建的宏基因组文库中分离的阳性蛋白酶细菌、选定的菌株和假阳性蛋白酶上进行了测试。

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