Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA.
Forensic Sci Int Genet. 2013 Jan;7(1):129-35. doi: 10.1016/j.fsigen.2012.07.008. Epub 2012 Aug 21.
As short tandem repeat markers remain the foundation of human identification throughout the world, new STR multiplexes require rigorous testing to ensure the assays are sufficiently robust and reliable for genotyping purposes. The PowerPlex(®) 18D System was created for the direct amplification of buccal and blood samples from FTA(®) storage cards and reliably accommodates other sample materials. The PowerPlex(®) 18D System allows simultaneous amplification of the 13 CODIS loci and amelogenin along with four additional loci: Penta E, Penta D, D2S1338, and D19S433. To demonstrate suitability for human identification testing, the PowerPlex(®) 18D System was tested for sensitivity, concordance, inhibitor tolerance, and performance with thermal cycling and reaction condition variation following SWGDAM developmental validation guidelines. Given these results, PowerPlex(®) 18D System can confidently be used for forensic and human identification testing.
由于短串联重复序列标记物仍然是全球人类身份识别的基础，因此新的 STR 多重扩增试剂盒需要经过严格的测试，以确保检测在基因分型方面足够强大和可靠。PowerPlex(®)18D 系统是为直接扩增来自 FTA(®)储存卡的口腔和血液样本而创建的，可可靠地容纳其他样本材料。PowerPlex(®)18D 系统允许同时扩增 13 个 CODIS 基因座和 amelogenin 基因，以及另外四个基因座：Penta E、Penta D、D2S1338 和 D19S433。为了证明其适合人类身份识别测试，PowerPlex(®)18D 系统按照 SWGDAM 开发验证指南进行了灵敏度、一致性、抑制剂耐受性以及热循环和反应条件变化的性能测试。鉴于这些结果，PowerPlex(®)18D 系统可以自信地用于法医和人类身份识别测试。
