Kraemer Melanie, Prochnow Anke, Bussmann Michael, Scherer Mario, Peist Ralf, Steffen Carolyn
QIAGEN GmbH, QIAGEN Strasse 1, 40724 Hilden, Germany.
QIAGEN GmbH, QIAGEN Strasse 1, 40724 Hilden, Germany.
Forensic Sci Int Genet. 2017 Jul;29:9-20. doi: 10.1016/j.fsigen.2017.03.012. Epub 2017 Mar 12.
The original CODIS database based on 13 core STR loci has been overwhelmingly successful for matching suspects with evidence. In order to increase the power of discrimination, reduce the possibility of adventitious matches, and expand global data sharing, the CODIS Core Loci Working Group determined the expansion of the CODIS core loci to 20 STR plus three additional "highly recommended" loci (SE33, DY391, Amelogenin) Hares, 2015, 2012 [1,2]. The QIAGEN Investigator 24plex QS and Investigator 24plex GO! Kits are 6-dye multiplex assays that contain all markers of the expanded 23 CODIS core loci along with a unique internal performance control that is co-amplified with the STR markers. The "Quality Sensor" generates additional information for quality control and performance checks. Investigator 24plex QS is designed for purified DNA from casework and reference samples, whereas 24plex GO! is dedicated to direct amplification of reference samples, like blood or buccal cells on FTA or swabs. A developmental validation study was performed on both assays. Here, we report the results of this study which followed the recommendations of the European Network of Forensic Science Institutes (ENFSI) [3] and the Revised Validation Guidelines of the Scientific Working Group on DNA Analysis Methods (SWGDAM) [4]. Data included are for PCR-based procedures e.g. reaction conditions, effects of PCR annealing temperature variations, amplification cycles or cyclers, sensitivity (also in the context of the Quality Sensor), performance with simulated inhibition, stability and efficiency, precision, reproducibility, mixture study, concordance, stutter, species specificity, and case-type samples. The validation results demonstrate that the Investigator 24plex QS and Investigator 24plex GO! Kits are robust and reliable identification assays as required for forensic DNA typing in forensic casework analysis and databasing.
基于13个核心短串联重复序列（STR）位点的原始联合DNA索引系统（CODIS）数据库在将嫌疑人与证据进行比对方面取得了巨大成功。为了提高鉴别能力、降低偶然匹配的可能性并扩大全球数据共享，CODIS核心位点工作组决定将CODIS核心位点扩展至20个STR加上另外三个“强烈推荐”的位点（SE33、DY391、牙釉蛋白）[1,2]。哈里斯，2015年，2012年。QIAGEN Investigator 24plex QS和Investigator 24plex GO!试剂盒是6染料多重分析方法，包含扩展后的23个CODIS核心位点的所有标记以及与STR标记共同扩增的独特内部性能对照。“质量传感器”为质量控制和性能检查提供额外信息。Investigator 24plex QS专为从案件样本和参考样本中提取的纯化DNA设计，而24plex GO!则专门用于直接扩增参考样本，如FTA或拭子上的血液或口腔细胞。对这两种分析方法都进行了开发验证研究。在此，我们报告该研究的结果，该研究遵循了欧洲法医学研究所网络（ENFSI）[3]和DNA分析方法科学工作组（SWGDAM）的修订验证指南[4]。所包含的数据适用于基于聚合酶链反应（PCR）的程序，例如反应条件、PCR退火温度变化的影响、扩增循环或扩增仪、灵敏度（也包括质量传感器方面）、模拟抑制下的性能、稳定性和效率、精密度、重现性、混合研究、一致性、拖尾、物种特异性以及案件类型样本。验证结果表明，Investigator 24plex QS和Investigator 24plex GO!试剂盒是法医案件分析和数据库建设中法医DNA分型所需的强大且可靠的鉴定分析方法。
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