State Key Laboratory of Agrobiotechnology and School of Life Sciences, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong Special Administrative Region.
J Plant Physiol. 2013 Jan 1;170(1):101-4. doi: 10.1016/j.jplph.2012.08.003. Epub 2012 Aug 24.
Soil salinization is a global issue that hampers agricultural production. Chloride is one of the prominent anions on saline land that cause toxicity to the plant. We previously identified the GmCLC1 gene from soybean (Glycine max) that encodes a putative tonoplast-localized chloride transporter. In this study, using electrophysiological analysis, we demonstrated the chloride transport function of GmCLC1. Interestingly, this chloride transport activity is pH dependent, suggesting that GmCLC1 is probably a chloride/proton antiporter. When the cDNA of GmCLC1 was expressed in tobacco BY-2 cells under the control of a constitutive promoter, the protective effect against salinity stress in transgenic tobacco BY-2 cells was also found to be pH sensitive. In the native host soybean, the expression of GmCLC1 gene is regulated by pH. All these findings support the notion that the function of GmCLC1 is regulated by pH.
土壤盐渍化是一个全球性问题，它会阻碍农业生产。氯离子是盐渍土中一种主要的阴离子，会对植物造成毒害。我们之前从大豆（Glycine max）中鉴定出一个氯离子转运蛋白基因 GmCLC1，该基因编码一个假定的液泡膜定位氯离子转运蛋白。在这项研究中，我们通过电生理分析证明了 GmCLC1 的氯离子转运功能。有趣的是，这种氯离子转运活性依赖于 pH 值，这表明 GmCLC1 可能是一种氯离子/质子反向转运蛋白。当 GmCLC1 的 cDNA 在烟草 BY-2 细胞中受组成型启动子的控制表达时，我们还发现转基因烟草 BY-2 细胞对盐胁迫的保护作用也对 pH 值敏感。在大豆的天然宿主中，GmCLC1 基因的表达受到 pH 值的调控。所有这些发现都支持 GmCLC1 的功能受 pH 值调控的观点。
