Department of Medicine and Public Health, Section of Human Anatomy, Second University of Naples, Italy.
Environ Pollut. 2012 Dec;171:162-7. doi: 10.1016/j.envpol.2012.07.006. Epub 2012 Aug 23.
In the recent literature there has been an increased interest in the effects of particulate matter on the respiratory tract. The objective of this study was to use an in vitro model of type II lung epithelium (A549) to evaluate the cell ability to take up sub-micron PM(1.0) particles (PM(1.0)), Parietaria officinalis (ALL), and PM(1.0) + ALL together. Morphological analysis performed by Transmission Electron Microscope (TEM) showed that PM and ALL interacted with the cell surface, then penetrating into the cytoplasm. Each single treatment was able to point out a specific change in the morphology. The cells treated appear healthy and not apoptotic. The main effect was the increase of: multilamellar bodies, lysosomal enzymes, microvilli, and presence of vesicle/vacuoles containing particles. These observations demonstrate morphological and functional alterations related to the PM(1.0) and P. officinalis and confirm the induction of the inflammatory response in lung cells exposed to the inhalable particles.
在最近的文献中，人们对颗粒物对呼吸道的影响越来越感兴趣。本研究的目的是使用 II 型肺上皮细胞（A549）的体外模型来评估细胞摄取亚微米 PM(1.0) 颗粒（PM(1.0)）、豚草（ALL）以及 PM(1.0) + ALL 的能力。透射电子显微镜（TEM）进行的形态分析表明，PM 和 ALL 与细胞表面相互作用，然后穿透细胞质。每种单一处理都能够指出细胞形态的特定变化。处理后的细胞看起来健康，没有凋亡。主要作用是增加：多层体、溶酶体酶、微绒毛以及含有颗粒的囊泡/空泡的存在。这些观察结果表明与 PM(1.0) 和豚草有关的形态和功能改变，并证实了暴露于可吸入颗粒物的肺细胞中炎症反应的诱导。
