Getz D, Gibson J F, Sheppard R N, Micklem K J, Pasternak C A
J Membr Biol. 1979 Nov 30;50(3-4):311-29. doi: 10.1007/BF01868895.
When Lettree cells are exposed to Mn2+, the cation becomes associated with cells in two ways: in a relatively loose and mobile manner that gives a six-line EPR spectrum designated Mnb*, and in an immobile, relatively tight manner that gives no detectable EPR specrtum, designated Mnb. Mnb* is probably on the surface of cells; most Mnb is probably inside cells. NMR measurements of Lettree cell suspensions show two water proton relaxation rates and confirm the existence of cell-associated Mn. Human erythrocytes, on the other hand, bind no Mn2+ under these conditions, as judged by EPR and NMR measurements. Virally-treated Lettree cells show an increase in Mnb (but not in Mnb*). They also show a third water proton relaxation rate.
当Lettre细胞暴露于Mn2+时，该阳离子以两种方式与细胞结合：一种是相对松散且可移动的方式，产生一条六线EPR谱线，称为Mnb*；另一种是不可移动、相对紧密的方式，不产生可检测到的EPR谱线，称为Mnb。Mnb可能位于细胞表面；大多数Mnb可能在细胞内部。对Lettre细胞悬液的核磁共振测量显示出两种水质子弛豫率，并证实了细胞相关锰的存在。另一方面，通过EPR和核磁共振测量判断，在这些条件下人类红细胞不结合Mn2+。经病毒处理的Lettre细胞显示Mnb增加（但Mnb不增加）。它们还显示出第三种水质子弛豫率。
