Subramanian Dharaneedharan, Ramalingam Rajkumar, Karuppasamy Radhakrishnan, Subramanian Thanga Leela, Chellam Balasundaram, Rajan Koilmani Emmanuvel
Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli, India.
Neuroreport. 2012 Oct 3;23(14):840-5. doi: 10.1097/WNR.0b013e328357bdbf.
In the present study, we observed variations in the expression pattern of proteins isolated from the telencephalon of goldfish (Carassius auratus). The expression of a 28 kDa protein was elevated in the individuals trained in a spatial task when compared with the untrained individuals. The ∼28 kDa protein was analyzed using liquid chromatography and mass spectrometry; further, the data were analyzed using the MASCOT search engine. The analysis showed that the ∼28 kDa protein is a β form of 14-3-3 protein with 35.1% identity. In addition, the semiquantitative PCR confirmed the variation in the expression of 14-3-3 between the trained and the untrained groups. Subsequently, we examined the effect of upregulation of 14-3-3 (β) in the neurotransmitters; that is, serotonin (5-hydroxytryptamine, 5-HT) and dopamine (DA). Notably, the level of 5-HT and DA was found to be significantly elevated in the telencephalon of individuals trained in the spatial task than in the untrained individuals. Our results suggest that the spatial learning increases the expression of 14-3-3 (β), which in turn leads to an increase in the level of 5-HT and DA. The upregulated 5-HT and DA may facilitate synapse formation during spatial learning in a novel environment.
在本研究中，我们观察了从金鱼（Carassius auratus）端脑分离的蛋白质表达模式的变化。与未训练的个体相比，在空间任务中训练的个体中一种28 kDa蛋白质的表达升高。使用液相色谱和质谱对约28 kDa的蛋白质进行了分析；此外，使用MASCOT搜索引擎对数据进行了分析。分析表明，约28 kDa的蛋白质是14-3-3蛋白质的β形式，同一性为35.1%。此外，半定量PCR证实了训练组和未训练组之间14-3-3表达的差异。随后，我们研究了神经递质中14-3-3（β）上调的影响；即血清素（5-羟色胺，5-HT）和多巴胺（DA）。值得注意的是，在空间任务中训练的个体端脑中5-HT和DA的水平明显高于未训练的个体。我们的结果表明，空间学习增加了14-3-3（β）的表达，进而导致5-HT和DA水平的增加。上调的5-HT和DA可能在新环境中的空间学习过程中促进突触形成。
