Vargas J P, Rodr¿iguez F, L¿opez J C, Arias J L, Salas C
Laboratorio de Psicobiolog¿ia, Universidad de Sevilla, Avda. San Francisco Javier, s/n, 41005-Sevilla, Spain.
Brain Res. 2000 May 19;865(1):77-84. doi: 10.1016/s0006-8993(00)02220-4.
Spatial learning and memory related morphological changes in the argyrophilic nucleolar organizer region (AgNOR) of telencephalic neurons in goldfish were quantitatively evaluated by means of AgNOR neurohistochemical stain. The AgNORs and nuclei of nerve cells of two different telencephalic regions of goldfish trained in a spatial task or submitted to a similar non-contingent behavioral procedure (control group) were morphometrically evaluated. Results show that the area of AgNORs in goldfish dorsolateral telencephalic neurons increased significantly in the spatial learning group but not in control group. This effect seems to be highly specific as it did not appear in the dorsolateral area of the control group neither in the dorsomedial area of both groups. As the size of AgNORs in the nerve cell nuclei reflect the level of transcriptive activity, these morphological changes could be revealing increased protein synthesis in goldfish dorsolateral telencephalic neurons related with learning and memory. These findings could contribute to determining the subregions of the teleost telencephalon implicated in spatial learning and could indicate that the AgNOR staining technique would be a useful tool in assesing learning and memory related neuronal activity.
通过银染核仁组成区（AgNOR）神经组织化学染色，对金鱼端脑神经元中与空间学习和记忆相关的形态变化进行了定量评估。对在空间任务中训练的金鱼或接受类似非关联行为程序（对照组）的金鱼两个不同端脑区域的神经细胞的AgNORs和细胞核进行了形态测量评估。结果表明，空间学习组金鱼背外侧端脑神经元中AgNORs的面积显著增加，而对照组没有。这种效应似乎具有高度特异性，因为在对照组的背外侧区域以及两组的背内侧区域均未出现。由于神经细胞核中AgNORs的大小反映转录活性水平，这些形态变化可能揭示了金鱼背外侧端脑神经元中与学习和记忆相关的蛋白质合成增加。这些发现可能有助于确定硬骨鱼端脑中与空间学习有关的亚区域，并可能表明AgNOR染色技术将是评估与学习和记忆相关的神经元活动的有用工具。
