Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, PR China.
Mol Med Rep. 2012 Nov;6(5):1103-6. doi: 10.3892/mmr.2012.1038. Epub 2012 Aug 16.
While a number of genetic and environmental risk factors for coronary heart disease (CHD) have been identified, the list of potential risk factors remains long. One candidate is dimethylarginine dimethylaminohydrolase (DDAH2), which is known to be polymorphic in humans. The gene product indirectly increases the endogenous production of nitric oxide, an anti-atherogenic molecule. Therefore, alterations in DDAH2 activity may indirectly result in an increased risk of CHD. We studied allele and genotype distributions for two polymorphic loci of DDAH2, rs805305 and rs2272592, in 180 patients with CHD and 180 healthy controls. Disease history and other clinical data were recorded. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to determine the genotype at rs805305, and ligase detection reaction (LDR) was used to determine the genotype at rs2272592. Systolic blood pressure and blood triglyceride and glucose levels were higher, and history of hypertension, diabetes, smoking and alcohol use was more common in the patients with CHD (P<0.05). However, the genotype and allele frequencies at the two polymorphic loci of DDAH2 were not statistically different between the two groups. Therefore, no association was observed between the DDAH2 polymorphisms at rs805305 and rs2272592 and CHD.
虽然已经确定了一些冠心病（CHD）的遗传和环境风险因素，但潜在风险因素的清单仍然很长。一个候选因素是二甲基精氨酸二甲氨基水解酶（DDAH2），已知其在人类中存在多态性。该基因产物间接增加了内源性一氧化氮的产生，一氧化氮是一种抗动脉粥样硬化的分子。因此，DDAH2 活性的改变可能会间接导致 CHD 风险增加。我们研究了 DDAH2 两个多态性位点 rs805305 和 rs2272592 的等位基因和基因型分布，在 180 例 CHD 患者和 180 例健康对照中进行了研究。记录了疾病史和其他临床数据。聚合酶链反应-限制性片段长度多态性（PCR-RFLP）用于确定 rs805305 的基因型，连接酶检测反应（LDR）用于确定 rs2272592 的基因型。CHD 患者的收缩压和血液甘油三酯和血糖水平较高，且高血压、糖尿病、吸烟和饮酒史更为常见（P<0.05）。然而，两个多态性位点 DDAH2 的基因型和等位基因频率在两组之间没有统计学差异。因此，DDAH2 多态性 rs805305 和 rs2272592 与 CHD 之间没有观察到关联。
