Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou, PR China.
Planta Med. 2012 Oct;78(15):1667-75. doi: 10.1055/s-0032-1315239. Epub 2012 Aug 24.
Four new steroidal saponins, pariposides A-D (1-4), and two new sterol glycosides, pariposides E-F (5-6), along with eight known steroidal saponins (7-14), two known sterol glycosides (15-16), and two known ecdysteroids (17-18), were isolated from the roots of Paris polyphylla var. yunnanensis. Among them, compounds 1-4 are the first spirostanol saponins with a peroxy group located between C-5 and C-8 of the aglycone. Their structures were determined by detailed spectroscopic analyses and chemical methods. All the isolated compounds were evaluated for their in vitro cytotoxicities against human nasopharyngeal carcinoma epithelial (CNE) cells, and steroidal saponins 7, 11, 13, and 14 showed a potent antiproliferative effect on CNE cells with IC₅₀ values of 9.2, 4.7, 11.1, and 2.7 µM, respectively.
从云南重楼的根部分离得到四个新的甾体皂甙，即 pariposides A-D（1-4），以及两个新的甾体糖苷，即 pariposides E-F（5-6），此外还有八个已知的甾体皂甙（7-14），两个已知的甾体糖苷（15-16）和两个已知的蜕皮甾体（17-18）。其中，化合物 1-4 是第一个具有过氧基位于糖苷元 C-5 和 C-8 之间的螺甾烷醇皂甙。通过详细的光谱分析和化学方法确定了它们的结构。所有分离得到的化合物都进行了体外细胞毒性评估，对人鼻咽上皮（CNE）细胞的抑制活性，甾体皂甙 7、11、13 和 14 对 CNE 细胞表现出强烈的增殖抑制作用，IC₅₀ 值分别为 9.2、4.7、11.1 和 2.7µM。
