Torkzaban P, Saffarpour A, Bidgoli M, Sohilifar S
Periodontology Department, Dental Research Center, Faculty of Dentistry, Hamedan University of medical science, Hamedan, Iran.
J Dent (Tehran). 2012 Winter;9(1):1-6. Epub 2012 Mar 31.
Stem cells are of great interest for regenerating disturbed tissues and organs. These cells are commonly isolated from the bone marrow, but there has been interest in other tissues in the recent years. In this study, we evaluated the possibility of isolation of stem cells from oral connective tissue and investigated their characteristics.
In this experimental study, sampling from the bone marrow and oral connective tissue of a beagle dog was performed under general anesthesia. Bone marrow stem cell isolation was performed according to the established protocols. The samples obtained from oral soft tissue were broken to small pieces and after adding collagenase I, the samples were incubated for 45 minutes in 37°C. Other processes were similar to the processes which were carried out on bone marrow cells. Then cell properties were compared to evaluate if the cells from the connective tissue were stem cells.
The cells from the bone marrow and connective tissue had the same morphology. The result of colony forming unit assay was relatively similar. Population doubling time was similar too. In addition, both cell groups differentiated to osteoblasts in osteogenic media.
The cells isolated from the oral connective tissue had the characteristics of stem cells, including fibroblastoid morphology, self renewal properties, high proliferation rate and differentiation potential.
干细胞对于再生受损组织和器官具有重大意义。这些细胞通常从骨髓中分离获取，但近年来人们对其他组织也产生了兴趣。在本研究中，我们评估了从口腔结缔组织中分离干细胞的可能性，并研究了它们的特性。
在本实验研究中，在全身麻醉下从一只比格犬的骨髓和口腔结缔组织中进行取样。按照既定方案进行骨髓干细胞的分离。从口腔软组织获取的样本被切成小块，加入Ⅰ型胶原酶后，样本在37℃下孵育45分钟。其他步骤与对骨髓细胞所进行的步骤相似。然后比较细胞特性，以评估结缔组织来源的细胞是否为干细胞。
骨髓来源的细胞和结缔组织来源的细胞具有相同的形态。集落形成单位检测结果相对相似。群体倍增时间也相似。此外，两个细胞组在成骨培养基中均分化为成骨细胞。
从口腔结缔组织中分离出的细胞具有干细胞的特性，包括成纤维细胞样形态、自我更新特性、高增殖率和分化潜能。
