Vado-Solís Ignacio A, Suárez-Solís Víctor, Jiménez-Delgadillo Bertha, Zavala-Velázquez Jorge E, Segura-Correa José C
Unidad Interinstitucional de Investigación Clínica Epidemiológica, Facultad de Medicina, Universidad Autónoma de Yucatán. Av. Itzáes entre 59 y 59-A Apdo. Postal 1225-A C.P. 97000, Mérida, Yucatán, México.
J Parasitol. 2013 Apr;99(2):383-5. doi: 10.1645/GE-3189.1. Epub 2012 Aug 27.
The objective of this study was to estimate the seroprevalence of anti- Toxoplasma gondii antibodies and DNA of women with spontaneous abortions in 2 hospitals located in Yucatan, Mexico. Between June 2008 and May 2009, blood samples were taken from 100 women with spontaneous abortion attending the Ticul City Communitarian Hospital and the Merida Mother-Kid Hospital. The sera were tested for anti- T. gondii IgG and IgM antibodies. Blood samples (5 ml with anticlotting agent) were also used for polymerase chain reaction (PCR) assay, to detect T. gondii DNA. Forty-two of the 100 samples were negative. Of the positive samples (n = 58), 32 were positive to IgG, 2 to IgM, 5 to IgG and IgM, 6 to IgG and PCR, 1 to IgM and PCR, and 12 to IgG, IgM, and PCR. Accordingly, 55% of the women were seropositive to at least IgG, 20% to at least IgM, and 19% via PCR. Differences between hospitals were significant (P < 0.05) only for IgM. The risk of infection (IgM positive) was 2.85 (odds ratio [OR] 95%, confidence interval [CI]; 1.03-7.87) times greater in women patients at the Merida Mother-Kid Hospital, than those at the Ticul Communitarian Hospital. More studies are needed to evaluate the impact of this disease and to establish strategies to follow in order to reduce congenital toxoplasmosis in the populations at risk.
本研究的目的是估计墨西哥尤卡坦半岛两家医院自然流产女性的抗弓形虫抗体血清阳性率及弓形虫DNA情况。2008年6月至2009年5月，从蒂库尔市社区医院和梅里达母婴医院的100名自然流产女性中采集血样。检测血清中的抗弓形虫IgG和IgM抗体。血样（5毫升含抗凝剂）也用于聚合酶链反应（PCR）检测，以检测弓形虫DNA。100份样本中有42份为阴性。在阳性样本（n = 58）中，32份IgG阳性，2份IgM阳性，5份IgG和IgM均阳性，6份IgG和PCR阳性，1份IgM和PCR阳性，12份IgG、IgM和PCR均阳性。因此，55%的女性至少IgG血清阳性，20%至少IgM血清阳性，19%通过PCR检测为阳性。仅IgM在两家医院之间的差异具有统计学意义（P < 0.05）。梅里达母婴医院女性患者的感染风险（IgM阳性）是蒂库尔社区医院女性患者的2.85倍（优势比[OR] 95%，置信区间[CI]；1.03 - 7.87）。需要更多研究来评估这种疾病的影响，并制定相应策略以降低高危人群中的先天性弓形虫病。
