埃及盖勒尤比省酶联免疫吸附测定法（ELISA）检测IgG、IgM及聚合酶链反应（PCR）诊断和研究弓形虫病的对比研究

Comparative study between elisa IgG, IgM and PCR in diagnosing and studying toxoplasmosis in Qualyobia Governorate, Egypt.

作者信息

el Fakahany Amany F, Abdel-Maboud Amina I, el-Garhy Manal F, Eraky Maysa A

机构信息

Department of Medical Parasitology, Faculty of Medicine, Benha Branch of Zagazig University, Benha, Egypt.

出版信息

J Egypt Soc Parasitol. 2002 Aug;32(2):475-86.

PMID:12214925

Abstract

ELISA IgG, IgM antibodies and PCR for toxoplasmosis were performed on 55 women with complicated gestation and their babies. Besides, ELISA IgG and IgM were applied on 27 uncomplicated gestation (mothers & babies) and 152 randomly selected individuals. Seropositivity to specific IgG antibodies was 36.4%, 59.2% and 57.9% and for IgM was 27.3%, 7.4% and 10.5% in complicated gestation. uncomplicated gestation and random population respectively. PCR was positive in 20%, 50% and 60% of mothers with abortion, premature deliveries and deliveries of babies with congenital anomalies respectively. 55.5% and 40% were found seropositive for IgG from normal full term babies and abnormal babies. 13% of abnormal babies were IgM positive and 46.6% were PCR positive from the same group.

摘要

对55名患有妊娠并发症的妇女及其婴儿进行了弓形虫病的ELISA IgG、IgM抗体检测和PCR检测。此外，对27名无并发症妊娠的妇女（母亲和婴儿）以及152名随机选取的个体进行了ELISA IgG和IgM检测。在患有妊娠并发症的妇女中，特异性IgG抗体血清阳性率分别为36.4%、59.2%和57.9%，IgM抗体血清阳性率分别为27.3%、7.4%和10.5%。在无并发症妊娠妇女和随机人群中，上述数据分别为……（此处原文未完整给出无并发症妊娠妇女和随机人群中IgG、IgM的具体数据）。流产母亲、早产母亲以及分娩出先天性异常婴儿的母亲中，PCR检测阳性率分别为20%、50%和60%。正常足月婴儿和异常婴儿中，IgG血清阳性率分别为55.5%和40%。同一组异常婴儿中，13%的婴儿IgM呈阳性，46.6%的婴儿PCR呈阳性。