富血小板血浆对犬下颌骨缺损新鲜冷冻同种异体骨骨愈合的影响：一项组织形态计量学研究

Effect of platelet-rich plasma on bone healing of fresh frozen bone allograft in mandibular defects: a histomorphometric study in dogs.

作者信息

Messora Michel R, Nagata Maria J H, Pola Natália M, de Campos Natália, Fucini Stephen E, Furlaneto Flávia A C

机构信息

Department of Surgery and Bucco-Maxillofacial Traumatology and Periodontology, Ribeirao Preto School of Dentistry, University of Sao Paulo - USP, Ribeirão Preto, SP, Brazil.

出版信息

Clin Oral Implants Res. 2013 Dec;24(12):1347-53. doi: 10.1111/clr.12008. Epub 2012 Aug 28.

DOI:10.1111/clr.12008

PMID:22924970

Abstract

OBJECTIVE

This study histologically analyzed the healing of fresh frozen bone allograft (FFBA) with or without platelet-rich plasma (PRP) in bony defects surgically created in the mandible of dogs.

MATERIAL AND METHODS

Bilateral resection defects were surgically created on the inferior border of the mandible in 10 dogs. The defects were randomly divided into three groups: C (the defect was filled by blood clot only), FFBA (the defect was filled with particulate FFBA), and FFBA/PRP (the defect was filled with particulate FFBA combined with PRP). The animals were euthanized at 12 weeks post-surgery. Histomorphometric and histological analyses were performed. Data were statistically analyzed using Friedman test and Dunn test (P < 0.05).

RESULTS

Group FFBA/PRP presented a statistically greater mineralized bone area and a smaller marrow bone area than Groups FFBA and C.

CONCLUSIONS

The use of PRP improved the incorporation of FFBA, increasing the density of newly formed bone at 12 weeks post-surgery.

摘要

目的

本研究通过组织学方法分析了在犬下颌骨手术制造的骨缺损中，使用或不使用富血小板血浆（PRP）的新鲜冷冻骨同种异体移植（FFBA）的愈合情况。

材料与方法

对10只犬的下颌骨下缘进行双侧手术切除缺损。缺损随机分为三组：C组（缺损仅用血凝块填充）、FFBA组（缺损用颗粒状FFBA填充）和FFBA/PRP组（缺损用颗粒状FFBA与PRP联合填充）。术后12周对动物实施安乐死。进行组织形态计量学和组织学分析。数据采用Friedman检验和Dunn检验进行统计学分析（P < 0.05）。