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莫洛尼氏鼠肉瘤病毒克隆3的RNA异源双链分析。

Heteroduplex analysis of the RNA of clone 3 Moloney murine sarcoma virus.

作者信息

Chien Y H, Verma I M, Duesberg P H, Davidson N

出版信息

J Virol. 1979 Dec;32(3):1028-32. doi: 10.1128/JVI.32.3.1028-1032.1979.

DOI:10.1128/JVI.32.3.1028-1032.1979
PMID:229257
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC525952/
Abstract

Heteroduplex analysis of the RNA isolated from purified virions of clone 3 Moloney murine sarcoma virus (M-MSV) hybridized to cDNA's from Moloney murine leukemia virus (M-MLV) and clone 124 M-MSV shows that the main physical component of clone 3 RNA is missing all or most of the 1.5-kilobase (kb) clone 124 M-MSV specific sequence denoted beta s (S. Hu et al. Cell 10:469--477, 1977). This sequence is either deleted in clone 3 RNA or substituted by a very short (0.3-kilobase) sequence. In other respects, clone 3 and clone 124 RNAs show the same heteroduplex structure relative to M-MLV. Since beta s is believed to contain the src gene(s) of clone 124 RNA, this result leaves as an unresolved question the nature of the src gene(s) of the clone 3 M-MSV RNA complex.

摘要

从克隆3莫洛尼鼠肉瘤病毒(M-MSV)纯化病毒粒子中分离的RNA与来自莫洛尼鼠白血病病毒(M-MLV)和克隆124 M-MSV的cDNA进行异源双链分析,结果表明克隆3 RNA的主要物理成分缺失了全部或大部分1.5千碱基(kb)的克隆124 M-MSV特异性序列,该序列称为βs(S. Hu等人,《细胞》10:469 - 477,1977)。此序列在克隆3 RNA中要么缺失,要么被一个非常短的(0.3千碱基)序列所取代。在其他方面,相对于M-MLV,克隆3和克隆124的RNA显示出相同的异源双链结构。由于βs被认为包含克隆124 RNA的src基因,该结果留下了一个未解决的问题,即克隆3 M-MSV RNA复合物的src基因的性质。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02b8/525952/4545ff3fc5ee/jvirol00192-0336-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02b8/525952/4545ff3fc5ee/jvirol00192-0336-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02b8/525952/4545ff3fc5ee/jvirol00192-0336-a.jpg

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本文引用的文献

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An electron microscope heteroduplex study of the ribosomal DNAs of Xenopus laevis and Xenopus mulleri.非洲爪蟾和穆氏爪蟾核糖体DNA的电子显微镜异源双链研究。
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辅助独立型鼠肉瘤病毒的证据。I. 复制缺陷型病毒和转化缺陷型病毒的分离
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A heteroduplex study of the sequence relationships between the RNAs of M-MSV and M-MLV.莫洛尼氏鼠肉瘤病毒(M-MSV)与莫洛尼氏鼠白血病病毒(M-MLV)RNA序列关系的异源双链研究
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An MSV-specific subgenomic mRNA in MSV-transformed G8-124 cells.在被MSV转化的G8 - 124细胞中一种MSV特异性亚基因组mRNA。
Cell. 1979 May;17(1):53-63. doi: 10.1016/0092-8674(79)90294-0.
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Heteroduplex analysis of the sequence relations between the RNAs of mink cell focus-inducing and murine leukemia viruses.水貂细胞灶诱导病毒与鼠白血病病毒RNA之间序列关系的异源双链分析。
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