莫洛尼氏鼠肉瘤病毒克隆3的RNA异源双链分析。
Heteroduplex analysis of the RNA of clone 3 Moloney murine sarcoma virus.
作者信息
Chien Y H, Verma I M, Duesberg P H, Davidson N
出版信息
J Virol. 1979 Dec;32(3):1028-32. doi: 10.1128/JVI.32.3.1028-1032.1979.
Heteroduplex analysis of the RNA isolated from purified virions of clone 3 Moloney murine sarcoma virus (M-MSV) hybridized to cDNA's from Moloney murine leukemia virus (M-MLV) and clone 124 M-MSV shows that the main physical component of clone 3 RNA is missing all or most of the 1.5-kilobase (kb) clone 124 M-MSV specific sequence denoted beta s (S. Hu et al. Cell 10:469--477, 1977). This sequence is either deleted in clone 3 RNA or substituted by a very short (0.3-kilobase) sequence. In other respects, clone 3 and clone 124 RNAs show the same heteroduplex structure relative to M-MLV. Since beta s is believed to contain the src gene(s) of clone 124 RNA, this result leaves as an unresolved question the nature of the src gene(s) of the clone 3 M-MSV RNA complex.
从克隆3莫洛尼鼠肉瘤病毒（M-MSV）纯化病毒粒子中分离的RNA与来自莫洛尼鼠白血病病毒（M-MLV）和克隆124 M-MSV的cDNA进行异源双链分析，结果表明克隆3 RNA的主要物理成分缺失了全部或大部分1.5千碱基（kb）的克隆124 M-MSV特异性序列，该序列称为βs（S. Hu等人，《细胞》10:469 - 477，1977）。此序列在克隆3 RNA中要么缺失，要么被一个非常短的（0.3千碱基）序列所取代。在其他方面，相对于M-MLV，克隆3和克隆124的RNA显示出相同的异源双链结构。由于βs被认为包含克隆124 RNA的src基因，该结果留下了一个未解决的问题，即克隆3 M-MSV RNA复合物的src基因的性质。
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