Donoghue D J, Sharp P A, Weinberg R A
Cell. 1979 May;17(1):53-63. doi: 10.1016/0092-8674(79)90294-0.
An intracellular subgenomic RNA species from MSV-transformed G8-124 cells was characterized by electron microscopy of RNA:cDNA heteroduplexes using long cDNAs both MSV and MuLV. This subgenomic RNA, 3.1 kb long, consisted of 5'-derived sequences of about 0.4 kb joined to 2.7 kb of RNA derived from the 3' end of the RNA genome. The 3'-derived sequences included the residual sequences from the MuLV pol region and the acquired cellular sequences of MSV. The genome of MSV was shown to retain approximately 0.13 kb from the 5' end of the MuLV env region, including sequences which span the point in the MuLV env mRNA. No subgenomic MSV RNA could be detected, however, which consisted of a 5'-derived leader sequence spliced to the retained env region sequences. Nor could a subgenomic MSV RNA be detected in which a 5'-derived leader sequence was joined directly to the acquired cellular sequences. Although its translation products are unknown, the subgenomic MSV RNA was present in preparations of poly(A)+ polysomal RNA, consistent with this RNA functioning as a messenger. The structure of this 3.1 kb MSV subgenomic RNA suggests a possible role in the expression of 3'-encoded MSV information, possibly including transformation-specific sequences.
利用源自MSV和MuLV的长链cDNA，通过RNA:cDNA异源双链体的电子显微镜观察，对MSV转化的G8 - 124细胞中的一种细胞内亚基因组RNA种类进行了表征。这种3.1 kb长的亚基因组RNA由约0.4 kb的5'端衍生序列与来自RNA基因组3'端的2.7 kb RNA连接而成。3'端衍生序列包括MuLV pol区域的残留序列和MSV获得的细胞序列。结果显示，MSV基因组从MuLV env区域的5'端保留了约0.13 kb，包括跨越MuLV env mRNA中某一点的序列。然而，未检测到由5'端衍生的前导序列与保留的env区域序列拼接而成的亚基因组MSV RNA。也未检测到5'端衍生的前导序列直接与获得的细胞序列连接的亚基因组MSV RNA。尽管其翻译产物尚不清楚，但这种亚基因组MSV RNA存在于聚腺苷酸加尾的多核糖体RNA制剂中，这与该RNA作为信使发挥功能一致。这种3.1 kb的MSV亚基因组RNA的结构表明其在3'端编码的MSV信息表达中可能发挥作用，可能包括转化特异性序列。
