Matsumura K, Hori S, Sugiura H, Shimizu T
Department of Neurology, Shimoshizu National Hospital, Chiba.
J Biochem. 1990 Oct;108(4):513-5. doi: 10.1093/oxfordjournals.jbchem.a123232.
Two-dimensional gel electrophoresis using 0.5% agarose gel in the 1st dimension and 2-12% gradient polyacrylamide gel in the 2nd dimension succeeded in the isoelectrical focusing of connectin, a giant myofibrillar protein of approximately 3,000 kDa. Immunoblotting with an anti-connectin monoclonal antibody, SM1-36-2, following the two-dimensional gel electrophoresis, demonstrated that connectin was an acidic protein with an estimated pI of approximately 5.7.
在一维使用0.5%琼脂糖凝胶、二维使用2-12%梯度聚丙烯酰胺凝胶的双向凝胶电泳成功实现了伴肌动蛋白（一种分子量约为3000 kDa的巨大肌原纤维蛋白）的等电聚焦。二维凝胶电泳后，用抗伴肌动蛋白单克隆抗体SM1-36-2进行免疫印迹，结果表明伴肌动蛋白是一种酸性蛋白，估计其等电点约为5.7。
