Leirskar J, Helgeland K
Scand J Dent Res. 1977 Sep;85(6):471-9. doi: 10.1111/j.1600-0722.1977.tb00580.x.
A cell culture method has been used to study the effect of zinc phosphate cement (De Trey's Zinc Zement Improved), zinc silicophosphate cement (Fluoro-Thin) and polycarboxylate cement (Durelon) on animal cells. Disks (20 x 1 mm) of the materials were placed in the center of plastic Petri dishes and subsequently incubated with human epithelial cells. Cell multiplication, medium pH and the release of cement constituents were measured. All three cements exhibited a cytotoxic effect, which was most pronounced in the cultures with zinc silicophosphate cement and polycarboxylate cement. The results also indicated that cell growth on the surface of the disks is a more sensitive indicator of cytotoxicity than cell growth around the disks. pH of the medium was only slightly affected in cultures with polycarboxylate cement, whereas a decrease was found in cultures with zinc phosphate cement and especially with zinc silicophosphate cement. A rapid release of phosphate was found in cultures with zinc silicophosphate cement. Zinc was released into the medium from disks of zinc phosphate cement, zinc silicophosphate cement and polycarboxylate cement--exceeding the toxicity level for the present cell line after 24 h. In cultures with zinc silicophosphate cement and polycarboxylate cement the release of fluoride reached toxic levels within the same time interval.
一种细胞培养方法已被用于研究磷酸锌水门汀（De Trey's Zinc Zement Improved）、硅磷酸锌水门汀（Fluoro-Thin）和聚羧酸锌水门汀（Durelon）对动物细胞的影响。将这些材料的圆盘（20×1毫米）放置在塑料培养皿的中心，随后与人上皮细胞一起孵育。测量细胞增殖、培养基pH值和水门汀成分的释放。所有三种水门汀均表现出细胞毒性作用，在硅磷酸锌水门汀和聚羧酸锌水门汀培养物中最为明显。结果还表明，圆盘表面的细胞生长比圆盘周围的细胞生长对细胞毒性更敏感。聚羧酸锌水门汀培养物中培养基的pH值仅受到轻微影响，而磷酸锌水门汀培养物尤其是硅磷酸锌水门汀培养物中pH值有所下降。在硅磷酸锌水门汀培养物中发现磷酸盐快速释放。锌从磷酸锌水门汀、硅磷酸锌水门汀和聚羧酸锌水门汀的圆盘中释放到培养基中——24小时后超过了当前细胞系的毒性水平。在硅磷酸锌水门汀和聚羧酸锌水门汀培养物中，氟化物的释放在相同时间间隔内达到毒性水平。
