尿抗生素 5-硝基-8-羟基喹啉（硝呋太尔）通过螯合铁和锌来减少铜绿假单胞菌生物膜的形成并诱导其分散。
The urinary antibiotic 5-nitro-8-hydroxyquinoline (Nitroxoline) reduces the formation and induces the dispersal of Pseudomonas aeruginosa biofilms by chelation of iron and zinc.
机构信息
Institute of Medical Microbiology, Jena University Hospital, Jena, Germany.
出版信息
Antimicrob Agents Chemother. 2012 Nov;56(11):6021-5. doi: 10.1128/AAC.01484-12. Epub 2012 Aug 27.
Abstract
Since cations have been reported as essential regulators of biofilm, we investigated the potential of the broad-spectrum antimicrobial and cation-chelator nitroxoline as an antibiofilm agent. Biofilm mass synthesis was reduced by up to 80% at sub-MIC nitroxoline concentrations in Pseudomonas aeruginosa, and structures formed were reticulate rather than compact. In preformed biofilms, viable cell counts were reduced by 4 logs at therapeutic concentrations. Complexation of iron and zinc was demonstrated to underlie nitroxoline's potent antibiofilm activity.
摘要
由于阳离子已被报道为生物膜的必需调节剂，我们研究了广谱抗菌剂和阳离子螯合剂硝呋太尔作为抗生物膜剂的潜力。在亚最低抑菌浓度的硝呋太尔浓度下，铜绿假单胞菌的生物膜大量合成减少了多达 80%，并且形成的结构是网状的而不是致密的。在预先形成的生物膜中，治疗浓度下的活菌计数减少了 4 个对数级。铁和锌的络合作用被证明是硝呋太尔具有强大抗生物膜活性的基础。
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本文引用的文献
1
Zinc fingered: new compounds that thwart gram-positive biofilm formation by sequestering zinc.锌指作用：通过螯合锌来阻止革兰氏阳性菌生物膜形成的新化合物。
Chembiochem. 2010 Apr 12;11(6):758-60. doi: 10.1002/cbic.200900691.
2
Increase in rhamnolipid synthesis under iron-limiting conditions influences surface motility and biofilm formation in Pseudomonas aeruginosa.在缺铁条件下，鼠李糖脂的合成增加会影响铜绿假单胞菌的表面运动性和生物膜形成。
J Bacteriol. 2010 Jun;192(12):2973-80. doi: 10.1128/JB.01601-09. Epub 2010 Feb 12.
3
In vitro effect of ultrasound on bacteria and suggested protocol for sonication and diagnosis of prosthetic infections.超声对细菌的体外作用及人工关节感染超声处理与诊断的建议方案
J Clin Microbiol. 2009 Aug;47(8):2496-501. doi: 10.1128/JCM.02316-08. Epub 2009 Jun 17.
4
Iron-binding compounds impair Pseudomonas aeruginosa biofilm formation, especially under anaerobic conditions.铁结合化合物会损害铜绿假单胞菌生物膜的形成，尤其是在厌氧条件下。
J Med Microbiol. 2009 Jun;58(Pt 6):765-773. doi: 10.1099/jmm.0.004416-0.
5
A zinc-dependent adhesion module is responsible for intercellular adhesion in staphylococcal biofilms.一种锌依赖性黏附模块负责葡萄球菌生物膜中的细胞间黏附。
Proc Natl Acad Sci U S A. 2008 Dec 9;105(49):19456-61. doi: 10.1073/pnas.0807717105. Epub 2008 Dec 1.
6
Activity of Pseudomonas aeruginosa in biofilms: effect of calcium.铜绿假单胞菌在生物膜中的活性：钙的影响。
Biotechnol Bioeng. 1989 Jan 20;33(4):406-14. doi: 10.1002/bit.260330405.
7
The iron stimulon of Xylella fastidiosa includes genes for type IV pilus and colicin V-like bacteriocins.桑氏假单胞菌的铁刺激基因包括IV型菌毛和类大肠杆菌素V细菌素的基因。
J Bacteriol. 2008 Apr;190(7):2368-78. doi: 10.1128/JB.01495-07. Epub 2008 Jan 25.
8
The assimilation of amino-acids by bacteria; trace metals in glutamic acid assimilation and their inactivation by 8-hydroxyquinoline.细菌对氨基酸的同化作用；谷氨酸同化过程中的痕量金属及其被8-羟基喹啉的失活作用
J Gen Microbiol. 1949 Sep;3(3):369-86. doi: 10.1099/00221287-3-3-369.
9
Urinary tract infections in patients with chronic renal insufficiency.慢性肾功能不全患者的尿路感染
Clin J Am Soc Nephrol. 2006 Mar;1(2):327-31. doi: 10.2215/CJN.01931105. Epub 2006 Jan 11.
10
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Environ Microbiol. 2007 Jul;9(7):1842-50. doi: 10.1111/j.1462-2920.2007.01286.x.
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