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Proteomic profile of the nucellus of castor bean (Ricinus communis L.) seeds during development.蓖麻（Ricinus communis L.）种子珠心在发育过程中的蛋白质组特征。
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Multidimensional strategy for sensitive phosphoproteomics incorporating protein prefractionation combined with SIMAC, HILIC, and TiO(2) chromatography applied to proximal EGF signaling.采用蛋白质预分级结合 SIMAC、HILIC 和 TiO(2)色谱的多维敏感磷酸蛋白质组学策略，应用于近 EGFR 信号转导。
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Identification of nitrotyrosine containing peptides using combined fractional diagonal chromatography (COFRADIC) and off-line nano-LC-MALDI.使用联合分馏对角色谱（COFRADIC）和离线纳升液相色谱-基质辅助激光解吸电离飞行时间质谱联用技术鉴定含硝基酪氨酸的肽段。
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Leaf-cutting ant fungi produce cell wall degrading pectinase complexes reminiscent of phytopathogenic fungi.切叶蚁真菌产生细胞壁降解果胶酶复合物，使人联想到植物病原真菌。
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基于质谱的蛋白质组学：背景、现状与未来需求。

Mass spectrometry based proteomics, background, status and future needs.

机构信息

Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230, Odense M, Denmark.

出版信息

Protein Cell. 2012 Sep;3(9):641-7. doi: 10.1007/s13238-012-2079-5. Epub 2012 Aug 29.

DOI:10.1007/s13238-012-2079-5
PMID:22926765
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4875375/
Abstract

An overview of the background for proteomics and a description of the present state of art are given with a description of the main strategies in proteomics. The advantages and limitations of the two major strategies, 2D-gel based and LC-MS based, are discussed and a combination for the two, CeLC-MS is described. A number of challenging problems which have been solved using different proteomics strategies including the advantage of organell enrichment or modifications specific peptide isolation to get deeper into the proteome are described. Finally the present status and future needs discussed.

摘要

概述了蛋白质组学的背景，并介绍了蛋白质组学的现状，描述了主要的蛋白质组学策略。讨论了基于 2D 凝胶和 LC-MS 两种主要策略的优缺点，并描述了它们的组合 CeLC-MS。描述了使用不同蛋白质组学策略解决的一些具有挑战性的问题，包括细胞器富集或修饰特异性肽分离的优势，以更深入地了解蛋白质组。最后讨论了当前的现状和未来的需求。