Blond McIndoe Laboratories, School of Biomedicine, The University of Manchester, Manchester Academic Health Science Centre, Oxford Road, Manchester M13 9PT, United Kingdom.
J Biomed Mater Res A. 2013 Feb;101(2):491-501. doi: 10.1002/jbm.a.34345. Epub 2012 Aug 28.
Cell-material interactions are crucial for cell adhesion and proliferation on biomaterial surfaces. Immobilization of biomolecules leads to the formation of biomimetic substrates, improving cell response. We introduced RGD (Arg-Gly-Asp) sequences on poly-ε-caprolactone (PCL) film surfaces using thiol chemistry to enhance Schwann cell (SC) response. XPS elemental analysis indicated an estimate of 2-3% peptide functionalization on the PCL surface, comparable with carbodiimide chemistry. Contact angle was not remarkably reduced; hence, cell response was only affected by chemical cues on the film surface. Adhesion and proliferation of Schwann cells were enhanced after PCL modification. Particularly, RGD immobilization increased cell attachment up to 40% after 6 h of culture. It was demonstrated that SC morphology changed from round to very elongated shape when surface modification was carried out, with an increase in the length of cellular processes up to 50% after 5 days of culture. Finally RGD immobilization triggered the formation of focal adhesion related to higher cell spreading. In summary, this study provides a method for immobilization of biomolecules on PCL films to be used in peripheral nerve repair, as demonstrated by the enhanced response of Schwann cells.
细胞-材料相互作用对于细胞在生物材料表面的黏附和增殖至关重要。生物分子的固定化导致仿生底物的形成，从而改善细胞反应。我们使用巯基化学将 RGD（精氨酸-甘氨酸-天冬氨酸）序列引入聚己内酯（PCL）薄膜表面，以增强施万细胞（SC）的反应。XPS 元素分析表明，PCL 表面的肽官能化估计为 2-3%，与碳二亚胺化学相当。接触角没有明显降低；因此，细胞反应仅受薄膜表面化学线索的影响。施万细胞在 PCL 修饰后黏附和增殖增强。特别是，RGD 固定化在培养 6 小时后将细胞附着增加了 40%。结果表明，当进行表面修饰时，SC 形态从圆形变为非常细长的形状，在培养 5 天后细胞突起的长度增加了 50%。最后，RGD 固定化触发了与更高细胞铺展相关的焦点粘连的形成。总之，这项研究提供了一种将生物分子固定在 PCL 薄膜上的方法，可用于周围神经修复，如施万细胞反应增强所证明的那样。
