• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

牙囊干细胞的成骨分化。

Osteogenic differentiation of dental follicle stem cells.

机构信息

Department of Biomedical Sciences, Medical School, University of Foggia, Italy.

出版信息

Int J Med Sci. 2012;9(6):480-7. doi: 10.7150/ijms.4583. Epub 2012 Aug 13.

DOI:10.7150/ijms.4583
PMID:22927773
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3427952/
Abstract

BACKGROUND

Stem cells are defined as clonogenic cells capable of self-renewal and multi-lineage differentiation. A population of these cells has been identified in human Dental Follicle (DF). Dental Follicle Stem Cells (DFSCs) were found in pediatric unerupted wisdom teeth and have been shown to differentiate, under particular conditions, into various cell types of the mesenchymal tissues.

AIM

The aim of this study was to investigate if cells isolated from DF show stem features, differentiate toward osteoblastic phenotype and express osteoblastic markers.

METHODS

We studied the immunophenotype of DFSCs by flow cytometric analysis, the osteoblastic markers of differentiated DFSCs were assayed by histochemical methods and real-time PCR.

RESULTS

We demonstrated that DFSCs expressed a heterogeneous assortment of makers associated with stemness. Moreover DFSCs differentiated into osteoblast-like cells, producing mineralized matrix nodules and expressed the typical osteoblastic markers, Alkaline Phosphatase (ALP) and Collagen I (Coll I).

CONCLUSION

This study suggests that DFSCs may provide a cell source for tissue engineering of bone.

摘要

背景

干细胞被定义为具有自我更新和多谱系分化能力的克隆细胞。在人类牙滤泡（DF）中已经鉴定出这些细胞的一个群体。牙滤泡干细胞（DFSCs）存在于儿童未萌出的智齿中，并且已经显示在特定条件下分化为间充质组织的各种细胞类型。

目的

本研究旨在探讨从 DF 分离出的细胞是否具有干细胞特征，能否向成骨表型分化，并表达成骨细胞标志物。

方法

我们通过流式细胞术分析研究了 DFSCs 的免疫表型，通过组织化学方法和实时 PCR 检测分化的 DFSCs 的成骨细胞标志物。

结果

我们证明了 DFSCs 表达与干细胞相关的异质标志物。此外，DFSCs 分化为成骨样细胞，产生矿化基质结节，并表达典型的成骨细胞标志物碱性磷酸酶（ALP）和 I 型胶原（Coll I）。

结论

本研究表明，DFSCs 可为骨组织工程提供细胞来源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45b1/3427952/0815f0b28ac8/ijmsv09p0480g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45b1/3427952/100c45a7d34a/ijmsv09p0480g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45b1/3427952/0c3ece1e9336/ijmsv09p0480g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45b1/3427952/d2d9f5ba4371/ijmsv09p0480g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45b1/3427952/c968325c0a9f/ijmsv09p0480g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45b1/3427952/0815f0b28ac8/ijmsv09p0480g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45b1/3427952/100c45a7d34a/ijmsv09p0480g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45b1/3427952/0c3ece1e9336/ijmsv09p0480g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45b1/3427952/d2d9f5ba4371/ijmsv09p0480g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45b1/3427952/c968325c0a9f/ijmsv09p0480g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45b1/3427952/0815f0b28ac8/ijmsv09p0480g05.jpg

相似文献

1
Osteogenic differentiation of dental follicle stem cells.牙囊干细胞的成骨分化。
Int J Med Sci. 2012;9(6):480-7. doi: 10.7150/ijms.4583. Epub 2012 Aug 13.
2
The role of dentin matrix protein 1 (DMP1) in regulation of osteogenic differentiation of rat dental follicle stem cells (DFSCs).牙本质基质蛋白1（DMP1）在大鼠牙囊干细胞（DFSCs）成骨分化调控中的作用。
Arch Oral Biol. 2015 Apr;60(4):546-56. doi: 10.1016/j.archoralbio.2014.12.013. Epub 2015 Jan 7.
3
Expression of odontogenic ameloblast-associated protein in the dental follicle and its role in osteogenic differentiation of dental follicle stem cells.牙源性成釉细胞相关蛋白在牙囊中的表达及其对牙囊干细胞成骨分化的作用。
Arch Oral Biol. 2017 Jun;78:6-12. doi: 10.1016/j.archoralbio.2017.02.001. Epub 2017 Feb 5.
4
TGF-β2 downregulates osteogenesis under inflammatory conditions in dental follicle stem cells.TGF-β2 在炎症条件下下调牙周膜干细胞的成骨作用。
Int J Oral Sci. 2018 Oct 9;10(3):29. doi: 10.1038/s41368-018-0028-8.
5
Activation of proliferation and differentiation of dental follicle stem cells (DFSCs) by heat stress.热应激激活牙囊干细胞的增殖和分化。
Cell Prolif. 2013 Feb;46(1):58-66. doi: 10.1111/cpr.12004. Epub 2012 Dec 21.
6
Expression of bone morphogenetic protein-6 in dental follicle stem cells and its effect on osteogenic differentiation.骨形态发生蛋白-6在牙囊干细胞中的表达及其对成骨分化的影响。
Cells Tissues Organs. 2013;198(6):438-47. doi: 10.1159/000360275. Epub 2014 Apr 8.
7
STRO-1 positive cell expansion during osteogenic differentiation: A comparative study of three mesenchymal stem cell types of dental origin.成骨分化过程中 Stro-1 阳性细胞的扩增：三种牙源性间充质干细胞类型的比较研究。
Arch Oral Biol. 2021 Feb;122:104995. doi: 10.1016/j.archoralbio.2020.104995. Epub 2020 Nov 26.
8
CA1 Modulates the Osteogenic Differentiation of Dental Follicle Stem Cells by Activating the BMP Signaling Pathway In Vitro.CA1 通过体外激活 BMP 信号通路调节牙滤泡干细胞的成骨分化。
Tissue Eng Regen Med. 2024 Aug;21(6):855-865. doi: 10.1007/s13770-024-00642-4. Epub 2024 Apr 23.
9
Gene expression of runx2, Osterix, c-fos, DLX-3, DLX-5, and MSX-2 in dental follicle cells during osteogenic differentiation in vitro.体外成骨分化过程中牙囊细胞中runx2、Osterix、c-fos、DLX-3、DLX-5和MSX-2的基因表达。
Calcif Tissue Int. 2006 Feb;78(2):98-102. doi: 10.1007/s00223-005-0146-0. Epub 2006 Feb 6.
10
Dental Follicle Stem Cells: Tissue Engineering and Immunomodulation.牙滤泡干细胞：组织工程与免疫调节。
Stem Cells Dev. 2019 Aug 1;28(15):986-994. doi: 10.1089/scd.2019.0012. Epub 2019 May 7.

引用本文的文献

1
Electrical Stimulation of Oral Tissue-Derived Stem Cells: Unlocking New Potential for Dental and Periodontal Regeneration.口腔组织来源干细胞的电刺激：开启牙齿与牙周再生的新潜能
Cells. 2025 Jun 4;14(11):840. doi: 10.3390/cells14110840.
2
CA1 induced dental follicle stem cells co-culture with dental pulp stem cells and loaded three-dimensional printed PCL/β-TCP scaffold: a novel strategy for alveolar cleft bone regeneration.CA1诱导牙囊干细胞与牙髓干细胞共培养并负载三维打印的PCL/β-TCP支架：一种牙槽裂骨再生的新策略。
J Mater Sci Mater Med. 2025 Apr 25;36(1):37. doi: 10.1007/s10856-025-06889-0.
3
Bone regeneration driven by a nano-hydroxyapatite/chitosan composite bioaerogel for periodontal regeneration.

本文引用的文献

1
Myeloma cells suppress osteoblasts through sclerostin secretion.骨髓瘤细胞通过分泌硬化蛋白抑制成骨细胞。
Blood Cancer J. 2011 Jun;1(6):e27. doi: 10.1038/bcj.2011.22. Epub 2011 Jun 24.
2
Dental pulp stem cells: osteogenic differentiation and gene expression.牙髓干细胞：成骨分化与基因表达。
Ann N Y Acad Sci. 2011 Nov;1237:47-52. doi: 10.1111/j.1749-6632.2011.06234.x.
3
Sclerostin is overexpressed by plasma cells from multiple myeloma patients.骨硬化蛋白在多发性骨髓瘤患者的浆细胞中过度表达。
纳米羟基磷灰石/壳聚糖复合生物气凝胶驱动的骨再生用于牙周再生
Front Bioeng Biotechnol. 2024 Jul 30;12:1355950. doi: 10.3389/fbioe.2024.1355950. eCollection 2024.
4
(c.G143A, p.S48L) Mutation Is Involved in Primary Failure of Eruption by Attenuating Glycolysis-Mediated Osteogenesis.(c.G143A，p.S48L) 突变通过削弱糖酵解介导的成骨作用导致出牙原发性失败。
Int J Mol Sci. 2024 Feb 29;25(5):2821. doi: 10.3390/ijms25052821.
5
MA Demethylase Inhibits Osteogenesis of Dental Follicle Stem Cells via Regulating miR-7974/FKBP15 Pathway.MA 去甲基化酶通过调控 miR-7974/FKBP15 通路抑制牙滤泡干细胞成骨分化。
Int J Mol Sci. 2023 Nov 9;24(22):16121. doi: 10.3390/ijms242216121.
6
Advancing Dentistry through Bioprinting: Personalization of Oral Tissues.通过生物打印推动牙科发展：口腔组织的个性化定制
J Funct Biomater. 2023 Oct 20;14(10):530. doi: 10.3390/jfb14100530.
7
Clinical application prospects and transformation value of dental follicle stem cells in oral and neurological diseases.牙囊干细胞在口腔及神经疾病中的临床应用前景与转化价值
World J Stem Cells. 2023 Apr 26;15(4):136-149. doi: 10.4252/wjsc.v15.i4.136.
8
Interactions between Dental MSCs and Biomimetic Composite Scaffold during Bone Remodeling Followed by In Vivo Real-Time Bioimaging.骨重建过程中仿生复合支架与牙间充质干细胞的相互作用及其体内实时生物成像研究。
Int J Mol Sci. 2023 Jan 17;24(3):1827. doi: 10.3390/ijms24031827.
9
Runx2 and Nell-1 in dental follicle progenitor cells regulate bone remodeling and tooth eruption.Runx2 和 Nell-1 在牙囊祖细胞中调节骨重塑和牙齿萌出。
Stem Cell Res Ther. 2022 Sep 30;13(1):486. doi: 10.1186/s13287-022-03140-3.
10
Potential of Bone-Marrow-Derived Mesenchymal Stem Cells for Maxillofacial and Periodontal Regeneration: A Narrative Review.骨髓间充质干细胞用于颌面部和牙周组织再生的潜力：一项叙述性综述
Int J Dent. 2021 Nov 9;2021:4759492. doi: 10.1155/2021/4759492. eCollection 2021.
Ann N Y Acad Sci. 2011 Nov;1237:19-23. doi: 10.1111/j.1749-6632.2011.06196.x.
4
Complete pulp regeneration after pulpectomy by transplantation of CD105+ stem cells with stromal cell-derived factor-1.经 CD105+ 干细胞联合基质细胞衍生因子-1 移植实现牙髓切除术后的完全牙髓再生。
Tissue Eng Part A. 2011 Aug;17(15-16):1911-20. doi: 10.1089/ten.TEA.2010.0615.
5
Finite element method (FEM), mechanobiology and biomimetic scaffolds in bone tissue engineering.有限元方法（FEM）、生物力学和仿生支架在骨组织工程中的应用。
Int J Biol Sci. 2011 Jan 26;7(1):112-32. doi: 10.7150/ijbs.7.112.
6
A network connecting Runx2, SATB2, and the miR-23a~27a~24-2 cluster regulates the osteoblast differentiation program.一个连接 Runx2、SATB2 和 miR-23a~27a~24-2 簇的网络调节成骨细胞分化程序。
Proc Natl Acad Sci U S A. 2010 Nov 16;107(46):19879-84. doi: 10.1073/pnas.1007698107. Epub 2010 Oct 27.
7
Efficacy of periodontal stem cell transplantation in the treatment of advanced periodontitis.牙周干细胞移植治疗重度牙周炎的疗效。
Cell Transplant. 2011;20(2):271-85. doi: 10.3727/096368910X519292. Epub 2010 Aug 18.
8
Osteogenic properties of human dental pulp stem cells.人牙髓干细胞的成骨特性。
J Biol Regul Homeost Agents. 2010 Apr-Jun;24(2):167-75.
9
Mesenchymal stem cells derived from dental tissues vs. those from other sources: their biology and role in regenerative medicine.源自牙组织的间充质干细胞与源自其他来源的间充质干细胞：它们的生物学特性及其在再生医学中的作用。
J Dent Res. 2009 Sep;88(9):792-806. doi: 10.1177/0022034509340867.
10
Osteoblast apoptosis in periodontal disease: role of TNF-related apoptosis-inducing ligand.牙周病中的成骨细胞凋亡：肿瘤坏死因子相关凋亡诱导配体的作用
Int J Immunopathol Pharmacol. 2009 Jan-Mar;22(1):95-103. doi: 10.1177/039463200902200111.