Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, United States of America.
PLoS One. 2012;7(8):e43804. doi: 10.1371/journal.pone.0043804. Epub 2012 Aug 23.
The conserved TREX complex, which contains UAP56, Aly, CIP29, and the multi-subunit THO complex, functions in mRNA export. Recently, several putative new components of the human TREX complex were identified by mass spectrometry. Here, we investigated the function of two of these, PDIP3 and ZC11A. Our data indicate that both of these proteins are components of a common TREX complex and function in mRNA export. Recently, we found that both CIP29 and Aly associate with the DEAD box helicase UAP56 and with the TREX complex in an ATP-dependent manner. We now show that this is also the case for PDIP3 and ZC11A. Thus, together with previous work, our data indicate that the TREX complex participates in multiple ATP-dependent interactions.
保守的 TREX 复合物，包含 UAP56、Aly、CIP29 和多亚基 THO 复合物，在 mRNA 输出中发挥作用。最近，通过质谱分析鉴定了几个人类 TREX 复合物的假定新成分。在这里，我们研究了其中两个成分 PDIP3 和 ZC11A 的功能。我们的数据表明，这两种蛋白质都是常见 TREX 复合物的组成部分，并在 mRNA 输出中发挥作用。最近，我们发现 CIP29 和 Aly 都与 DEAD 盒解旋酶 UAP56 以及依赖 ATP 的 TREX 复合物相互作用。我们现在表明，PDIP3 和 ZC11A 也是如此。因此，结合以前的工作，我们的数据表明 TREX 复合物参与了多种依赖 ATP 的相互作用。
