Chellat Djalila, Rezgoune Mohamed Larbi, Hamane Douadi, Semmame Ouarda, Benlatrèche Cherifa, Abadi Noureddine, Satta Dalila
Faculty of Natural and Life Sciences, Department of Animal Biology, Laboratory of Biology and Molecular Genetics, University Mentouri Constantine, Constantine, Algeria.
Genet Test Mol Biomarkers. 2012 Aug;16(8):874-8. doi: 10.1089/gtmb.2011.0367.
The C677T allele of the methylenetetrahydrofolate reductase (MTHFR) gene has been suggested to represent a risk factor for male infertility. To confirm this association, the distribution of the single-nucleotide polymorphism C677T was investigated in idiopathic infertile Algerian patients with nonobstructive azoospermia (NOA) or severe oligoasthenoteratozoospermia (OAT). A case-control study was carried out, including 74 idiopathic infertile Algerian patients with NOA (n=46) or severe OAT (n=28) and 84 fertile men as controls. Polymorphism C677T was studied by polymerase chain reaction-restriction fragment length polymorphism, and the results were statistically analyzed.
The frequency of genotypes MTHFR 677CC, 677CT, and 677TT in idiopathic infertile men with NOA was 43.48%, 41.30%, and 15.22%; 39.29%, 50%, and 10.71% regarding the severe oligozoospermic men; and 42.86%, 45.24%, and 11.90% in the control group.
The data suggest that the C677T MTHFR polymorphism is not a risk factor for idiopathic male subfertility in an Algerian population.
亚甲基四氢叶酸还原酶（MTHFR）基因的C677T等位基因被认为是男性不育的一个风险因素。为了证实这种关联，我们调查了患有特发性非梗阻性无精子症（NOA）或严重少弱畸精子症（OAT）的阿尔及利亚不育患者中，单核苷酸多态性C677T的分布情况。开展了一项病例对照研究，纳入74名患有特发性不育的阿尔及利亚患者，其中46例为NOA患者，28例为严重OAT患者，另有84名有生育能力的男性作为对照。通过聚合酶链反应-限制性片段长度多态性研究C677T多态性，并对结果进行统计学分析。
患有NOA的特发性不育男性中，MTHFR 677CC、677CT和677TT基因型的频率分别为43.48%、41.30%和15.22%；严重少精子症男性中分别为39.29%、50%和10.71%；对照组中分别为42.86%、45.24%和11.90%。
数据表明，在阿尔及利亚人群中，C677T MTHFR多态性不是特发性男性生育力低下的风险因素。
