Ueda F, Watanabe M, Hirata Y, Kimura K
Research Laboratories, Nippon Shinyaku Co., Ltd., Kyoto, Japan.
J Pharmacol Methods. 1990 Dec;24(4):277-84. doi: 10.1016/0160-5402(90)90012-a.
The gastric mucosal content of prostaglandin (PG) E2, 6-keto PGF1 alpha, and thromboxane (TX) B2 was determined by radioimmunoassay in unfed rats. The stomach was removed, and gastric mucosal specimens were prepared by microwave irradiation of the 1) intact stomach, 2) frozen stomach, 3) separated frozen glandular portion, 4) frozen gastric mucosa separated from the stomach wall, and 5) separation of frozen gastric mucosa with no microwave irradiation. Procedure 1 resulted in PGE2, TXB2, and 6-keto PGF1 alpha levels of 0.75, 2.33, and 3.04 ng/g tissue in the fundus, and 0.33, 1.92, and 1.76 ng/g tissue in the antrum, respectively. In procedures 2-4 these values were much higher, indicating that the PG content of the gastric mucosa is liable to be changed by various artificial procedures, such as freezing and surgical and mechanical handling. In procedure 5, values of PG contents were quite unreliable. These results suggest that microwave irradiation immediately after removal of the stomach may be the most reliable procedure for the accurate determination of the PG content of the gastric mucosa in vivo.
采用放射免疫分析法测定了未进食大鼠胃黏膜中前列腺素（PG）E2、6-酮-前列腺素F1α和血栓素（TX）B2的含量。取出胃，通过对以下样本进行微波照射来制备胃黏膜标本：1）完整胃；2）冷冻胃；3）分离的冷冻腺体部分；4）从胃壁分离的冷冻胃黏膜；5）未进行微波照射的冷冻胃黏膜分离样本。方法1测得胃底组织中PGE2、TXB2和6-酮-前列腺素F1α的含量分别为0.75、2.33和3.04 ng/g，胃窦组织中分别为0.33、1.92和1.76 ng/g。在方法2 - 4中，这些值要高得多，这表明胃黏膜中的PG含量容易因各种人工操作而改变，如冷冻、手术和机械处理。在方法5中，PG含量值非常不可靠。这些结果表明，胃切除后立即进行微波照射可能是在体内准确测定胃黏膜PG含量的最可靠方法。
