印度恶性疟原虫种群中富含组氨酸蛋白的遗传变异：导致疟疾快速诊断检测敏感性可变的可能原因。
Genetic variation in histidine rich proteins among Indian Plasmodium falciparum population: possible cause of variable sensitivity of malaria rapid diagnostic tests.
机构信息
National Institute of Malaria Research (ICMR), Sector 8, Dwarka, New Delhi, 110077, India.
出版信息
Malar J. 2012 Aug 28;11:298. doi: 10.1186/1475-2875-11-298.
Abstract
BACKGROUND
Rapid diagnostic tests (RDTs) have revolutionized the diagnosis of malaria. Among the various factors affecting RDTs sensitivity is genetic variation of the antigen used. The genetic variation in PfHRP2 and PfHRP3 proteins was studied among the Indian Plasmodium falciparum isolates.
METHODS
One hundred and forty isolates of P. falciparum were collected from six geographical regions of India. Target genes encoding PfHRP2 and PfHRP3 antigens were sequenced to study genetic polymorphism. Minimum detection limit giving a positive rapid diagnostic test was also determined.
RESULTS
Extensive variations were observed in amino acid repeat types of PfHRP2 and PfHRP3. PfHRP2 exhibited more polymorphism than PfHRP3. Significant relation was observed between type 2 and type 7 repeats and RDT detection rate as higher number of these repeats showed better sensitivity with RDTs.
CONCLUSION
The results provide insights into the genetic diversity of Pfhrp2 and Pfhrp3 genes among Indian P. falciparum population and its relation to RDT sensitivity.
摘要
背景
快速诊断检测（RDT）已经彻底改变了疟疾的诊断方式。在影响 RDT 敏感性的各种因素中，使用的抗原的遗传变异是一个重要因素。本研究旨在研究印度间日疟原虫分离株中 PfHRP2 和 PfHRP3 蛋白的遗传变异。
方法
从印度六个地区采集了 140 株疟原虫分离株。对编码 PfHRP2 和 PfHRP3 抗原的靶基因进行测序，以研究遗传多态性。还确定了产生阳性快速诊断检测结果的最低检测限。
结果
PfHRP2 和 PfHRP3 的氨基酸重复类型观察到广泛的变异。PfHRP2 比 PfHRP3 表现出更多的多态性。重复类型 2 和 7 与 RDT 检测率之间存在显著关系，因为这些重复类型的数量越多，与 RDT 的敏感性越好。
结论
研究结果提供了印度间日疟原虫种群中 Pfhrp2 和 Pfhrp3 基因的遗传多样性及其与 RDT 敏感性的关系的深入了解。
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