疟原虫乳酸脱氢酶种内和种间遗传变异性的评估。
Evaluation of the intra- and inter-specific genetic variability of Plasmodium lactate dehydrogenase.
作者信息
Talman Arthur M, Duval Linda, Legrand Eric, Hubert Véronique, Yen Seiha, Bell David, Le Bras Jacques, Ariey Frédéric, Houze Sandrine
机构信息
Unité d'Epidémiologie Moléculaire, Institut Pasteur in Cambodia 5 Boulevard Monivong BP983, Phnom Penh, Kingdom of Cambodia.
出版信息
Malar J. 2007 Oct 25;6:140. doi: 10.1186/1475-2875-6-140.
BACKGROUND
Malaria diagnosis is vital to efficient control programmes and the recent advent of malaria rapid diagnostic tests (RDTs) provides a reliable and simple diagnostic method. However a characterization of the efficiency of these tests and the proteins they detect is needed to maximize RDT sensitivity.
METHODS
Plasmodial lactate dehydrogenase (pLDH) gene of wild isolates of the four human species of Plasmodium from a variety of malaria endemic settings were sequenced and analysed.
RESULTS
No variation in nucleotide was found within Plasmodium falciparum, synonymous mutations were found for Plasmodium malariae and Plasmodium. vivax; and three different types of amino acid sequence were found for Plasmodium ovale. Conserved and variable regions were identified within each species.
CONCLUSION
The results indicate that antigen variability is unlikely to explain variability in performance of RDTs detecting pLDH from cases of P. falciparum, P. vivax or P. malariae malaria, but may contribute to poor detection of P. ovale.
背景
疟疾诊断对于有效的控制计划至关重要，而疟疾快速诊断测试（RDT）的近期出现提供了一种可靠且简单的诊断方法。然而，需要对这些测试的效率及其检测的蛋白质进行表征，以最大限度地提高RDT的灵敏度。
方法
对来自各种疟疾流行地区的四种人类疟原虫野生分离株的疟原虫乳酸脱氢酶（pLDH）基因进行测序和分析。
结果
恶性疟原虫内未发现核苷酸变异，间日疟原虫和三日疟原虫发现了同义突变；卵形疟原虫发现了三种不同类型的氨基酸序列。在每个物种中都鉴定出了保守区和可变区。
结论
结果表明，抗原变异性不太可能解释检测恶性疟原虫、间日疟原虫或三日疟原虫病例中pLDH的RDT性能差异，但可能导致卵形疟原虫检测效果不佳。
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